This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sall, A. A.
Right arrow Articles by Mathiot, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sall, A. A.
Right arrow Articles by Mathiot, C.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 713-715, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.713-715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Reverse Transcriptase PCR in Early Diagnosis of Rift Valley Fever

A. A. Sall,1* E. A. Macondo,1 O. K. Sène,1 M. Diagne,1 R. Sylla,1 M. Mondo,1 L. Girault,1 L. Marrama,1 A. Spiegel,1 M. Diallo,1 M. Bouloy,2 and C. Mathiot1

Institut Pasteur de Dakar, Dakar, Senegal,,1 Institut Pasteur, Groupe des bunyaviridés, 75724 Paris Cedex 15, France2

Received 7 August 2001/ Returned for modification 5 October 2001/ Accepted 30 December 2001

Reverse transcriptase PCR (RT-PCR) for diagnosis of Rift Valley fever (RVF) was evaluated by using 293 human and animal sera sampled during an RVF outbreak in Mauritania in 1998. Results of the RT-PCR diagnostic method were compared with those of virus isolation (VI) and detection of immunoglobulin M (IgM) antibodies. Our results showed that RT-PCR is a specific, sensitive tool for RVF diagnosis in the early phase of the disease and that its results do not differ significantly from those obtained by VI. Moreover, the combined results of RT-PCR and IgM antibody detection were in 100% concordance with the results of VI.

* Corresponding author. Mailing address: Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. Phone: 221.839.92.23. Fax: 221.839.92.10. E-mail: sall{at}pasteur.sn.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 713-715, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.713-715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»