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Clinical and Diagnostic Laboratory Immunology, May 2002, p. 723-725, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.723-724.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Development, Evaluation, and Application of Lateral-Flow Immunoassay (Immunochromatography) for Detection of Rotavirus in Bovine Fecal Samples{dagger}

Yousif Al-Yousif, Joe Anderson, Cindy Chard-Bergstrom, and Sanjay Kapil*

Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506

Received 4 December 2001/ Returned for modification 6 February 2002/ Accepted 2 March 2002

A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, respectively, with both being 100% specific. When the LAT was the reference test, the LFT was 75% sensitive and 91% specific. Additional specimens (n = 45) were tested by the LFT and LAT alone, and results were identical for both methods.

* Corresponding author. Mailing address: Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4457. Fax: (785) 532-4829. E-mail: kapil{at}vet.ksu.edu.

{dagger} Contribution 01-436-J from the Kansas Agricultural Experiment Station, Manhattan.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 723-725, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.723-724.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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