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Clinical and Diagnostic Laboratory Immunology, July 2002, p. 927-930, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.927-930.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Microimmunoassay Using a Protein Chip: Optimizing Conditions for Protein Immobilization

Seung-Yong Seong*

Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Disease, Seoul National University Medical Research Center, Chongno-gu, Seoul 110-799, Republic of Korea

Received 30 July 2001/ Returned for modification 15 February 2002/ Accepted 18 April 2002

Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.

* Corresponding author. Present address: Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Disease, National Institute of Health, 4 Center Dr., Bldg. 4, Room 111, Bethesda, MD 20892. Phone: (301) 402-3447. Fax: (301) 496-0877. E-mail: sseong{at}niaid.nih.gov.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 927-930, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.927-930.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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