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Clinical and Diagnostic Laboratory Immunology, September 2002, p. 959-965, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.959-965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Peripheral Blood Lymphocyte Subsets in Adolescents: a Longitudinal Analysis from the REACH Project

Bret J. Rudy,1* Craig M. Wilson,2 Stephen Durako,3 Anna-Barbara Moscicki,4 Larry Muenz,3 and Steven D. Douglas1*

Children's Hospital of Philadelphia, Department of Pediatrics, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania,1 Division of Geographic Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama,2 Westat, Rockville, Maryland,3 Division of Adolescent Medicine, University of California at San Francisco, San Francisco, California4

Received 7 January 2002/ Returned for modification 25 February 2002/ Accepted 30 April 2002

Flow cytometry analysis of lymphocyte subset markers was performed for a group of sexually active, human immunodeficiency virus (HIV)-negative adolescents over a 2-year period to establish normative data. Data were collected in the REACH Project (Reaching for Excellence in Adolescent Care and Health), a multicenter, longitudinal study of HIV-positive and high-risk HIV-negative adolescents. Two- and three-color flow cytometry data were collected every 6 months for these subjects. We determined the effects of gender, race, and age on the following lymphocyte subset markers: total CD4+ cells, CD4+ naïve cells, CD4+ memory cells, all CD8+ cells, CD8+ naïve cells, CD8+ memory cells, CD16+ natural killer cells, and CD19+ B cells. Gender was the demographic characteristic most frequently associated with differences in lymphocyte subset measures. Females had higher total CD4+ cell and CD4+ memory cells counts and lower CD16+ cell counts than males. Age was associated with higher CD4+ memory cell counts as well as higher CD8+ memory cell counts. For CD19+ cells, there was an interaction between age and gender, with males having significantly lower CD19+ cell counts with increasing age, whereas there was no age effect for females. Race and/or ethnicity was associated with differences in total CD8+ cell counts and CD8+ memory cell counts, although both of these associations involved an interaction with gender.

* Corresponding author. Mailing address: Division of Adolescent Medicine, The Children's Hospital of Philadelphia, 34th and Civic Center Blvd., Philadelphia, PA 19104. Phone: (215) 590-1468. Fax: (215) 590-4708. E-mail for Bret J. Rudy: rudy{at}email.chop.edu. E-mail for Steven D. Douglas: douglas{at}email.chop.edu.

Clinical and Diagnostic Laboratory Immunology, September 2002, p. 959-965, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.959-965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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