This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ananieva, O. Articles by Wadström, T. Search for Related Content PubMed PubMed Citation Articles by Ananieva, O. Articles by Wadström, T.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1160-1164, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1160-1164.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immune Responses to Bile-Tolerant Helicobacter Species in Patients with Chronic Liver Diseases, a Randomized Population Group, and Healthy Blood Donors

Department of Immunology, University of Tartu, 51014 Tartu, Estonia,¹ Department of Medical Microbiology, Dermatology and Infection, University of Lund, 223 62 Lund, Sweden²

Received 15 February 2002/ Returned for modification 30 May 2002/ Accepted 22 July 2002

Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (ELISA). The results were compared with the antibody responses to Helicobacter pylori. For analysis of a possible cross-reactivity between bile-tolerant Helicobacter species and H. pylori, sera from a subpopulation of each group were absorbed with a whole-cell extract of H. pylori and retested by ELISA. Results before absorption showed that the mean value of the ELISA units for H. pullorum was significantly higher in patients with CLD than in healthy blood donors (P = 0.01). Antibody reactivity to cell surface protein of H. hepaticus was also significantly higher in the CLD patients than in the healthy blood donors and the population group (P = 0.005 and P = 0.002, respectively). Following the absorption, antibody responses to H. pullorum decreased significantly in all three groups (P = 0.0001 for CLD patients, P = 0.0005 for the population group, and P < 0.0001 for the blood donors), indicating that cross-reactivity between H. pylori and other Helicobacter spp. occurs. The antibody responses to H. hepaticus and H. bilis in CLD patients remained high following absorption experiments compared to ELISA results before absorption. The significance of this finding requires further investigations.

This article has been cited by other articles:

• Feng, S., Ku, K., Hodzic, E., Lorenzana, E., Freet, K., Barthold, S. W. (2005). Differential Detection of Five Mouse-Infecting Helicobacter Species by Multiplex PCR. CVI 12: 531-536 [Abstract] [Full Text]  
• Feng, S., Kendall, L. V., Hodzic, E., Wong, S., Lorenzana, E., Freet, K., Ku, K. S., Luciw, P. A., Barthold, S. W., Khan, I. H. (2004). Recombinant Helicobacter bilis Protein P167 for Mouse Serodiagnosis in a Multiplex Microbead Assay. CVI 11: 1094-1099 [Abstract] [Full Text]  
• Young, V. B., Knox, K. A., Pratt, J. S., Cortez, J. S., Mansfield, L. S., Rogers, A. B., Fox, J. G., Schauer, D. B. (2004). In Vitro and In Vivo Characterization of Helicobacter hepaticus Cytolethal Distending Toxin Mutants. Infect. Immun. 72: 2521-2527 [Abstract] [Full Text]  
• Nilsson, I., Kornilovs'ka, I., Lindgren, S., Ljungh, A., Wadstrom, T. (2003). Increased prevalence of seropositivity for non-gastric Helicobacter species in patients with autoimmune liver disease. J Med Microbiol 52: 949-953 [Abstract] [Full Text]