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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1165-1168, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1165-1168.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phagocytosis and Killing of Bacteria by Professional Phagocytes and Dendritic Cells

Markus Nagl,^1* Laco Kacani,¹ Brigitte Müllauer,¹ Eva-Maria Lemberger,¹ Heribert Stoiber,¹ Georg M. Sprinzl,² Harald Schennach,³ and Manfred P. Dierich¹

Institute of Hygiene and Social Medicine,¹ Department of Otorhinolaryngology, Leopold-Franzens-University of Innsbruck,² Central Institute for Blood Transfusion, General Hospital, Innsbruck, Austria³

Received 18 January 2001/ Returned for modification 16 April 2002/ Accepted 2 July 2002

Dendritic cells (DC) represent a class of professional antigen-presenting cells whose primary function is to alert the immune system, not to clear invading microorganisms. The objective of our study was to compare the abilities of polymorphonuclear neutrophilic leukocytes (PMN), monocytes, monocyte-derived macrophages (MDM), monocyte-derived immature DC (imDC), and mature DC (maDC) to ingest and destroy Staphylococcus aureus and Escherichia coli. Acridine orange staining and fluorescence microscopy demonstrated that MDM, followed by monocytes, imDC, and PMN, internalized bacteria well but that maDC exhibited less pronounced phagocytic activity. PMN, monocytes, and MDM exhibited a much higher capacity to kill ingested bacteria than both imDC and maDC. In summary, these data are in agreement with the generally accepted idea that different types of leukocytes fulfill specialized tasks in antigen presentation and killing of pathogens.

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* Corresponding author. Mailing address: Institute of Hygiene and Social Medicine, Leopold-Franzens-University of Innsbruck, Fritz-Pregl-Str. 3, A-6010 Innsbruck, Austria. Phone: 43 512 507 3430. Fax: 43 512 507 2870. E-mail: m.nagl{at}uibk.ac.at.

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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1165-1168, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1165-1168.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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