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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1229-1234, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1229-1234.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cytokine Profiles in Peripheral Blood Mononuclear Cells and Lymph Node Cells from Piglets Infected In Utero with Porcine Reproductive and Respiratory Syndrome Virus

B. Aasted,1* P. Bach,1 J. Nielsen,2 and P. Lind3

The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Kalvehave DK-4771,1 Danish Veterinary Institute,2 Danish Veterinary Institute, DK-1790 Copenhagen V, Copenhagen, Denmark3

Received 25 March 2002/ Returned for modification 12 May 2002/ Accepted 10 July 2002

The aim of the present study was to investigate at 2, 4, and 6 weeks after birth cytokine expression by peripheral blood mononuclear cells and bronchial lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus (PRRSV). Technically, by flow cytometry we were able to measure gamma interferon ({gamma}-IFN), tumor necrosis factor alpha (TNF-{alpha}), interleukin-4 (IL-4), and IL-8 levels. In general, we found increases in the percentages of IL-4-, {gamma}-IFN-, and TNF-{alpha}-producing lymphocytes in the infected piglets compared to the percentages in the uninfected control animals, while there was a decrease in the percentage of IL-8-producing monocytes. We believe that these findings reflect a general lymphocyte activation stage that is created due to the infection and that occurs in combination with impairment of the monocyte function, possibly due to the ongoing viral replication in these cells. Single-cell bronchial lymph node preparations exhibited very much the same cytokine profiles as peripheral blood mononuclear cells except for a lack of IL-8 production. When the levels of the individual cytokines in the three groups of PRRSV-infected piglets were compared, the levels of cytokine expression at 4 weeks diverged from those at 2 and 6 weeks, in that there was a significant decrease in the numbers of lymphocytes producing {gamma}-IFN and TNF-{alpha}. This tendency was also observed among blood monocytes and lymph node macrophages. Possible reasons for this temporary immunosuppression in the piglets at 4 weeks are discussed.

* Corresponding author. Mailing address: Laboratory of Virology and Immunology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbojlen 7, DK-1870 Frederiksberg C, Copenhagen, Denmark. Phone: 45 35282727. Fax: 45 35282742. E-mail: bas{at}kvl.dk.

Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1229-1234, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1229-1234.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Diaz, I., Darwich, L., Pappaterra, G., Pujols, J., Mateu, E. (2005). Immune responses of pigs after experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 86: 1943-1951 [Abstract] [Full Text]  
  • Suradhat, S., Thanawongnuwech, R. (2003). Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 84: 2755-2760 [Abstract] [Full Text]  
  • Jensen, P. V., Castelruiz, Y., Aasted, B. (2003). Cytokine Profiles in Adult Mink Infected with Aleutian Mink Disease Parvovirus. J. Virol. 77: 7444-7451 [Abstract] [Full Text]  


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