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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1392-1395, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1392-1395.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Vitro Detection of Apoptosis in Monocytes/Macrophages Infected with Human Coronavirus

Arlene R. Collins^*

Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214

Received 24 April 2002/ Returned for modification 17 July 2002/ Accepted 22 August 2002

Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 10^3.5 to 10⁶ 50% tissue culture-infective doses (TCID₅₀)/ml, and HCoV strain OC43 titers were only 10^1.2 to 10^2.7 TCID₅₀/ml.

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* Mailing address: Virology Laboratory, Department of Microbiology, School of Medicine and Biomedical Sciences, 3435 Main St., State University of New York at Buffalo, Buffalo, NY 14214. Phone: (716) 829-2161. Fax: (716) 829-2158. E-mail: acollins{at}acsu.buffalo.edu.

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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1392-1395, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1392-1395.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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