CVI Accepts, published online ahead of print on 4 November 2009

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Clin. Vaccine Immunol. doi:10.1128/CVI.00374-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Use of a Baculovirus-Expressed Structural Protein for the Detection of Antibodies to Foot-and-Mouth Disease Virus Type A by a Blocking Enzyme-Linked Immunosorbent Assay

Young-Joon Ko^*, Hyang-Sim Lee, Hye-Young Jeoung, Eun-Jeong Heo, Hyo-Rim Ko, Byung-Sik Chang, Hoo-Don Joo, U. Gerelmaa, B. Dashzeveg, S. Tserendorj, R. Sodnomdarjaa, Jong-Hyeon Park, Chang-Hee Kweon, In-Soo Cho, and Sang-Gi Paik

National Veterinary Research and Quarantine Service, Anyang, Gyeonggi, 430-824, South Korea; Department of Research and Development Laboratory, Jenobiotech Inc., Chuncheon, Gangwon, South Korea; States Central Veterinary Laboratory, Zaisan, Khan-Uul District, Ulaanbaatar 210153, Mongolia; and Department of Biological Science, Chungnam National University, Daejeon, South Korea

^* To whom correspondence should be addressed. Email: koyj{at}nvrqs.go.kr.

A blocking enzyme-linked immunosorbent assay using baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with cutoff of 53% inhibition. Its sensitivity was comparable to the virus neutralization test and LPB ELISA, indicating its potential as an alternative assay.