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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 904-908, Vol. 7, No. 6
Savyon Diagnostics Ltd., Ashdod
77610,1 Clinical Microbiology
Laboratory, Bnai Zion Medical Center, Haifa
31048,2 and Clinical Virology Unit,
Hadassah Medical Center, Jerusalem,3 Israel
Received 17 March 2000/Returned for modification 13 June
2000/Accepted 15 August 2000
Recently a few new herpes simplex virus (HSV) type-specific
serological diagnostic tests have been introduced to the commercial market, but these tests have some limitations. Moreover, it is not yet
clear which commercial test can be regarded as a "gold standard"
for the serodiagnosis of HSV infections. In order to improve the
clinical diagnostic value of serological tests for the detection of HSV
infections, we developed novel, competition-based enzyme-linked
immunosorbent assays for the specific determination of HSV type 2 antibodies (SeroHSV2) and HSV type 1 antibodies (SeroHSV1) and two
complementary tests for the detection of HSV immunoglobulin M (IgM) and
IgG antibodies (SeroHSV IgM and SeroHSV IgG). These four new kits were
evaluated in comparison with some commercial kits for the detection of
HSV antibodies that are commonly used at present in Israeli clinical
laboratories. The results indicate that SeroHSV2 is highly sensitive
(>92%) and highly specific (>94%). SeroHSV2 does not cross-react
with other alphaherpesvirus antibodies. SeroHSV1 is highly sensitive
(>94%) and specific (>91%) compared to four commercial available
kits. SeroHSV IgM is highly specific (>92%) in comparison with other
commercial HSV IgM tests. The sensitivity of SeroHSV IgM ranges between
50 and 70% compared to these tests. Further investigation of the
discrepant results obtained by using in-house competition tests
indicated that SeroHSV IgM is more sensitive. SeroHSV IgG was also
found to be highly sensitive (>94%) and highly specific (>92%)
compared to the other commercial HSV IgG tests.
Herpes simplex virus (HSV) is one of
the most widespread viruses known to cause acute and recurrent
infections in humans. Once acquired, HSV infects the sensory nerve
cells innervating the mucosal areas involved in the acute infection,
migrates to the regional sensory ganglion, and remains latent. When
active, the virus may cause the development of vesicles and ulcers
(4, 6).
There are two types of HSV. HSV type 1 (HSV-1, also known as
herpesvirus labialis) most commonly infects the oral region, causing
"cold sores." HSV-2 (commonly known as herpesvirus genitalis) most
often infects the genital and perianal regions. Each of the two types
can cause infections in the sites with which the other is more commonly
associated and can also infect other organs such as the skin, the eyes,
and the brain (6). Most HSV-infected persons may shed the
virus periodically even in the absence of clinical manifestations
(1, 13).
Seroepidemiologic studies have shown that about 80% of adults
worldwide have antibodies to HSV-1. Serological assays however, do not
indicate the site of infection. Therefore, it is difficult to define
what proportion of HSV-1-seropositive individuals has genital HSV-1 or
orolabial infections. Several studies suggest that the rate of genital
HSV-1 infection, like the rate of HSV-2 infection, is increasing
(10).
In recent years there has been a tremendous increase in the reported
incidences of genital herpes; HSV-2 seroprevalence in the United States
is estimated between 20 and 25% by the age of 40 years. Genital herpes
is more prevalent among women than among men. In patients with sexually
transmitted diseases, the seroprevalence may reach 50% (5,
8). Approximately 85% of women, and nearly all men, with
symptomatic acquisition of genital HSV-2 infection will have acute
recurrent episodes within the first year at an average rate of four to
five times per year (2). The tendency toward recurrent
episodes is greater with genital HSV-2 infection than with HSV-1
(13).
The chronic nature of this disease results in persistent psychosocial
or psychosexual distress for many patients. Patients undergoing
chemotherapy, organ transplant recipients, and patients with human
immunodeficiency virus (HIV) infection who suffer from episodes of
HSV-1 or -2 can develop severe and extensive lesions (1).
The most severely affected population is neonates, who acquire HSV
infection after exposure to the virus during delivery (3).
Numerous studies have revealed that the majority of HSV-2 infections
are undiagnosed. It is also estimated that about 20% of patients with
HSV-2 antibodies are truly asymptomatic or have lesions only in
locations such as the cervix, which are difficult to observe
(5).
Definitive diagnosis of genital herpes infections is fundamental to the
management of patients and the development of strategies to prevent
transmission to partners and neonates. Clinical presentation alone is
often insufficient to diagnose genital herpes. Detection of
type-specific antibodies is an important and essential part of accurate
diagnosis, even in silent carriers of HSV-2 infections.
Recently, a few new type-specific serodiagnostic tests have been
introduced to the market (1, 7, 9). In order to improve the
clinical diagnostic value of the serodiagnosis of HSV infections, we
have developed a highly specific and sensitive screening tool for the
determination of HSV-1 and -2 antibodies as well as complementary tests
for the detection of immunoglobulin M (IgM) and IgG antibodies.
SeroHSV diagnostic kits. (i) SeroHSV1 and SeroHSV2.
SeroHSV1
and SeroHSV2 (Savyon Diagnostics Ltd., Ashdod, Israel) are
semiquantitative competitive binding enzyme-linked immunosorbent assays
(ELISA) intended for the specific detection and determination of HSV-1
and HSV-2 antibodies in human serum samples. SeroHSV1 and SeroHSV2
microtiter plates are coated with partially purified virus proteins
from HSV1 (Macintyre strain) and HSV2 (G strain).
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Novel Approach for Specific Detection of Herpes Simplex Virus
Type 1 and 2 Antibodies and Immunoglobulin G and M Antibodies
ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
(ii) SeroHSV IgM. SeroHSV IgM (Savyon Diagnostics Ltd.) is a qualitative ELISA intended for the specific detection of IgM antibodies to HSV-1 and/or -2 viruses in human serum.
SeroHSV IgM microtiter plates are coated with partially purified virus proteins from HSV-1 and HSV-2. The test was performed as described in the manufacturer's instruction manual. Human serum samples were prediluted 1:105 with a ready-to-use serum diluent containing a stripping solution that eliminates IgG interference. A 50-µl volume comprising the ready-to-use cutoff serum (run in duplicate) and either the negative control, positive control, or prediluted serum sample was added to a precoated microtiter plate and incubated 1 h at 37°C. Microtiter wells were washed three times with wash buffer. Then 50 µl of 1:300-diluted anti-human IgM antibodies conjugated to HRP was added and incubated 1 h at 37°C. Microtiter wells were washed three times with wash buffer, and then 100 µl of TMB substrate was added and incubated 15 min at room temperature. Subsequently, 100 µl of stop solution was added and absorbance (OD) was read at 450 and 620 nm. Test results were calculated and interpreted according to the instruction manual as follows. The average OD value of the cutoff serum run in duplicate was calculated. Then the cutoff index (COI) of each serum sample was determined by dividing the OD obtained for that serum sample by the average OD of the cutoff serum. A COI below or equal to 1 was considered negative, a COI above 1.1 was considered positive, and a COI between 1 and 1.1 was considered borderline.(iii) SeroHSV IgG. SeroHSV IgG (Savyon Diagnostics Ltd.) is a semiquantitative ELISA intended for the specific detection of IgG antibodies to HSV-1 and/or -2 viruses in human serum.
SeroHSV IgG microtiter plates are coated with partially purified virus proteins from HSV-1 and HSV-2. The test was performed as described in the manufacturer's instruction manual. Human serum samples, the positive control, and the negative control were prediluted 1:105 with a ready-to-use serum diluent. A 50-µl volume of each of the ready-to-use calibrators (calibrating solutions containing antibodies with binding capacities of 10, 50, or 100 arbitrary binding units [BU]/ml, referred to, respectively, as P10, P50, and P100 calibrators), diluted serum samples and controls were added to HSV-1- and HSV-2-precoated microtiter plates, and incubated 1 h at 37°C. Microtiter wells were washed three times with wash buffer. Then 50 µl of 1:300-diluted anti-human IgG antibodies conjugated to HRP was added and incubated 1 h at 37°C. Microtiter wells were washed three times with wash buffer, and then 100 µl of TMB substrate was added and incubated 15 min at room temperature. Subsequently, 100 µl of stop solution was added and the absorbance (OD) was determined at 450 and 620 nm. The results were calculated and interpreted according to the instruction manual. The OD values of the three calibrators (P100, P50, and P10) were plotted on the y axis against their respective concentrations (100 BU/ml for P100, 50 BU/ml for P50, and 10 BU/ml for P10) on the x axis. Then the best-fitted linear curve was drawn and was used for the interpolation of the concentration (in arbitrary binding units per milliliter) of each of the tested serum samples. Results below 10 BU/ml were considered negative, and those above 10 BU/ml were considered positive.Samples. Serum samples were obtained from four different Israeli clinical laboratories. Each of the laboratories precharacterized and preselected the samples utilizing the routinely used commercial serodiagnostic HSV kits. The samples were sent to Savyon Diagnostics and were tested blindly by the SeroHSV diagnostic tests. Sensitivity and specificity were determined relative to the results obtained by the different commercial kits described below.
The HSV Type 2-Specific IgG ELISA (Gull Laboratories Inc.) is a qualitative and quantitative ELISA intended for the specific detection of HSV-2 IgG antibodies in human serum samples. Affinity-purified HSV-2 glycoprotein G (gG2, MS strain) is used as the antigen in this test. The HSV Type 1-Specific IgG ELISA (Gull Laboratories Inc.) is a qualitative and quantitative ELISA intended for the specific detection of HSV-1 IgG antibodies in human serum samples. Affinity-purified HSV-1 glycoprotein G (gG1, MS strain) is used as the antigen in this test. The BioELISA HSV-2 IgG (Biokit, S.A., Barcelona, Spain) is a qualitative ELISA intended to detect HSV-2-specific IgG antibodies in human serum samples. Purified HSV-2 glycoprotein gG2 is used as the antigen in this test. The BioELISA HSV-1 IgG (Biokit, S.A.) is a qualitative ELISA intended to detect HSV-1-specific IgG antibodies in human serum samples. Purified HSV-1 glycoprotein gG1 is used as the antigen in this test. The HSV-1 and HSV-2 Differentiation Immunoblot IgG (MRL Diagnostics) is intended for use on serum samples previously determined to be positive for HSV antibodies. The MRL immunoblot test utilizes purified HSV and recombinant antigens (gG1 and gG2) immobilized on a nitrocellulose membrane. The ETI-HSVK-G2 (Sorin Biomedica Diagnostics S.P.A., Saluggia, Italy) is an ELISA for the qualitative detection of HSV-2 IgG antibodies in human serum samples. In this test HSV-2 recombinant glycoprotein gG2 is used as the antigen. The HSV 1+2 IgM ELISA (Gull Laboratories Inc.) is a qualitative IgM ELISA intended for the detection of IgM antibodies to HSV-1 and/or -2 in human serum samples. In this test, partially purified HSV (MacIntyre and St. Joer strains) antigens are used. The Enzygnost Anti-HSV/IgG (Dade Behring Marburg GmbH, Marburg, Germany) is an ELISA for the quantitative determination of human IgG antibodies to HSV in serum. In this test the antigen is derived from permanent simian kidney cells infected with HSV. The Enzygnost Anti-HSV/IgM (Dade Behring Marburg GmbH) is an ELISA for the detection of human IgM antibodies to HSV in serum. In this test the antigen is derived from permanent simian kidney cells infected with HSV. The Herpes Simplex Virus I ELISA (Genzyme Virotech GmbH, Rüsselsheim, Germany) is an ELISA for semiquantitative and qualitative detection of IgG and IgM antibodies to HSV-1 in human serum. Serum samples for the detection of IgM were pretreated by RF-SorboTech for the removal of excessive IgG levels. The Herpes Simplex Virus II ELISA (Genzyme Virotech GmbH) is an ELISA for semiquantitative and qualitative detection of IgG and IgM antibodies to HSV-2 in human serum. Serum samples for the detection of IgM were pretreated by RF-SorboTech for the removal of excessive IgG levels. The Anti-HSV-1 and -2 IgM Enzyme Immunoassay Kit (Diasorin, Sorin Diagnostics s.r.l., Saluggia, Italy) is a qualitative ELISA for the detection of IgM antibodies to HSV-1 and/or -2 in human serum. In this test HSV-1 and HSV-2 antigens were used.Relative sensitivity and specificity of SeroHSV2. Four sets of precharacterized serum samples derived from four different clinical laboratories and evaluated by four different commercial tests were tested blindly on the Savyon SeroHSV2 kit. The four different commercial tests detect specific human IgG antibodies to HSV-2 and utilize either purified or recombinant glycoprotein gG2 as the antigen.
The following serum samples were tested with the SeroHSV2 kit: (i) 244 serum samples precharacterized by the HSV Type 2-Specific IgG ELISA (Gull Laboratories Inc.), by which 71 samples were interpreted as positive and 173 as negative; (ii) 99 samples precharacterized by the ETI-HSVK-G2 (Sorin Biomedica Diagnostics S.P.A.), by which 7 samples were interpreted as positive and 92 as negative; (iii) 69 samples precharacterized by the HSV-1 and HSV-2 Differentiation Immunoblot IgG (MRL Diagnostics), by which 16 samples were interpreted as HSV-2 IgG positive and 53 were interpreted as HSV-2 IgG negative; and (iv) 82 samples precharacterized by the BioELISA HSV-2 IgG (Biokit, S.A.), by which 6 samples were interpreted as positive and 76 were interpreted as negative.Relative sensitivity and specificity of SeroHSV1. Four sets of precharacterized serum samples derived from four different clinical laboratories and evaluated by four different commercial tests were tested blindly with the Savyon SeroHSV1 kit. Three of the four different commercial tests (from Gull Laboratories Inc., Sorin Biomedica Diagnostics S.P.A., and MRL Diagnostics) detect specific human IgG antibodies to HSV-1 and utilize either purified or recombinant glycoprotein gG1 as the antigen. The fourth test detects specific IgG antibodies to HSV (Dade Behring). Most of the serum samples tested with this kit originated from patients at high risk of infection with HSV-1.
The following serum samples were tested with the SeroHSV1 kit: (i) 113 samples precharacterized by the HSV Type 1-Specific IgG ELISA (Gull Laboratories Inc.), by which 69 samples were interpreted as positive and 44 as negative; (ii) 53 samples precharacterized by the ETI-HSVK-G1 (Sorin Biomedica Diagnostics S.P.A.), by which 38 samples were interpreted as positive and 15 as negative; (iii) 98 samples precharacterized by the HSV-1 and HSV-2 Differentiation Immunoblot IgG (MRL Diagnostics), by which 77 samples were interpreted as HSV-1 IgG positive and 21 were interpreted as HSV-1 IgG negative; and (iv) 147 samples precharacterized by the Enzygnost Anti/HSV IgG (Dade Behring Marburg GmbH), by which 98 samples were interpreted as positive and 49 as negative.Relative sensitivity and specificity of SeroHSV IgM. To evaluate the relative sensitivity and specificity of SeroHSV IgM, four sets of serum samples precharacterized by four different commercial tests and derived from four different clinical laboratories were tested blindly with the Savyon SeroHSV IgM kit.
The following serum samples were tested with the SeroHSV IgM kit: (i) 61 samples precharacterized by the HSV 1+2 IgM ELISA (Gull Laboratories Inc.), by which 26 samples were interpreted as HSV IgM positive and 35 were interpreted as negative; (ii) 30 samples precharacterized by the Herpes Simplex Virus II ELISA Genzyme Virotech GmbH), by which 4 samples were interpreted as HSV IgM positive and 26 were interpreted as HSV IgM negative; (iii) 155 samples precharacterized by the Enzygnost Anti-HSV/IgM (Dade Behring Marburg GmbH), by which 10 samples were HSV IgM positive and 144 were HSV IgM negative; and (iv) 244 samples precharacterized by the Anti-HSV-1 and -2 IgM Enzyme Immunoassay Kit (Diasorin, Sorin Diagnostics s.r.l.), by which 16 samples were HSV IgM positive and 228 were HSV IgM negative.Evaluation of discrepant IgM results by competition assay. The SeroHSV IgM test was performed as described in the manufacturer's instruction manual with one alteration: the serum samples were prediluted 1:105 with or without the presence of either HSV-1 and -2 antigens or Mycoplasma pneumoniae antigens at a concentration 10 times higher than that of the HSV antigens with which the microtiter plates were coated. With true-positive results, the excess of free HSV antigens will compete with the immobilized HSV antigens on the specific HSV antibodies. If, however, the results are false positives, no competition should be observed. M. pneumoniae antigens served as a control to show that the competition was due to the specific antigen and was not due to any excess antigen.
Relative sensitivity and specificity of SeroHSV IgG. To evaluate the sensitivity and specificity of SeroHSV IgG, four sets of serum samples derived from four different clinical laboratories were precharacterized by nine different commercial tests and were tested blindly with the Savyon SeroHSV IgG kit.
The following serum samples were tested with the SeroHSV IgG kit: (i) 120 samples precharacterized by the HSV Type 2-Specific IgG ELISA and the HSV Type 1-Specific IgG ELISA (Gull Laboratories Inc.), by which 81 samples were interpreted as HSV positive and 39 were interpreted as negative; (ii) 51 samples precharacterized by the BioELISA HSV-2 IgG and the BioELISA HSV-1 IgG (Biokit, S.A.), by which 38 samples were interpreted as HSV positive and 13 were interpreted as HSV negative; (iii) 39 samples precharacterized by the HSV-1 and HSV-2 Differentiation Immunoblot IgG (MRL Diagnostics), by which 32 samples were interpreted as HSV positive and 7 were interpreted as HSV negative; (iv) 150 samples precharacterized by the Enzygnost Anti-HSV/IgG (Dade Behring Marburg GmbH), by which 100 samples were HSV IgG positive and 50 were HSV IgG negative; and (v) 138 samples precharacterized by the SeroHSV1 & SeroHSV2 tests (Savyon Diagnostics Ltd.), by which 93 samples were interpreted as HSV positive and 43 were interpreted as negative.| |
RESULTS |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
|
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Relative sensitivity and specificity of SeroHSV2.
In an
attempt to determine the sensitivity and specificity of any kit, one
needs to first define the "gold standard." Since there is no
agreement yet regarding which test is to be considered the "gold
standard" for the specific detection of HSV-2 antibodies, we have
evaluated the sensitivity and specificity of SeroHSV2 in comparison to
results obtained with the kits routinely used in Israeli clinical
laboratories. The relative sensitivity and specificity of SeroHSV2 in
comparison with each of the commercial kits are presented in Table
1.
|
SeroHSV2 and cross-reactivity.
The cross-reactivity of
SeroHSV2 with other alphaherpesviruses was determined by using the
following samples: (i) 68 serum samples preselected as HSV-1 IgG
positive and HSV-2 negative by the HSV Type 1- and HSV Type 2-Specific
IgG ELISAs (Gull Laboratories Inc.), (ii) 86 samples that were
varicella-zoster virus (VZV) IgG positive by the Enzygnost Anti-VZV/IgG
(Dade Behring), and (iii) 17 samples that were VZV IgG positive and HSV
IgG negative (Dade Behring). The specificity of SeroHSV2 as observed
with these serum samples is summarized in Table
2.
|
Relative sensitivity and specificity of SeroHSV1.
Seroepidemiology studies have shown high prevalence (>80%) of HSV-1
IgG antibodies among the normal population. This might influence the
results obtained with different HSV tests. Moreover, it is not yet
clear what is the serological "gold standard" assay for accurate
determination of HSV-1 antibodies. Therefore, results obtained with the
new competitive ELISA-based test, SeroHSV1, were compared to results
obtained with three different commercial tests (which claim to detect
HSV-1 IgG antibodies) and to results obtained with a test that detects
HSV IgG antibodies (Dade Behring) (Table
3).
|
Relative sensitivity and specificity of SeroHSV IgM compared to
different commercial kits.
In the absence of agreement as to which
commercial test can be regarded as the "gold standard" for the
detection of HSV IgM antibodies, the sensitivity and specificity of
SeroHSV IgM was determined in comparison with the results obtained from
tests currently used in several Israeli clinical laboratories (Table 4).
|
|
Relative sensitivity and specificity of SeroHSV IgG. To evaluate the sensitivity and specificity of the SeroHSV IgG kit, we decided to compare the results obtained by SeroHSV IgG with results obtained with other commercial kits that are in routine use in Israeli clinical laboratories.
Based on the results obtained with SeroHSV2 on the above four sets of serum samples, the relative sensitivity and specificity of SeroHSV IgG were calculated in comparison either to combinations of results obtained with commercial HSV-1- and HSV-2-specific serological kits (Gull Laboratories Inc., Biokit, S.A., MRL, and Savyon Diagnostics Ltd.) or to results obtained with one test that detected HSV IgG antibodies (Dade Behring), as summarized in Table 6.
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DISCUSSION |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Most of the new HSV IgG type-specific tests are based on either specific recombinant or purified native gG1 and gG2 proteins. In spite of the high specificities achieved with these tests, cross-reactions might be observed. Homology analysis reveals a 38% sequence identity between gG1 and gG2 proteins. Moreover, the purity level of the recombinant and the purification of gG proteins might affect the specificity. Sensitivity might also be lower either due to the lack of posttranslation epitopes in recombinant proteins or due to the late appearance of HSV IgG antibodies (2 to 3 months postinfection) (1). Detection of IgM antibodies might shorten the time needed to detect seroconversion.
Using a competition-based ELISA such as SeroHSV1 or SeroHSV2 minimizes these problems. Both tests are highly specific, since these tests are based on the ability of human antibodies to compete with MAbs against highly specific major immunodominant epitopes. No adverse impact on sensitivity was observed as determined by comparison to the other HSV IgG type-specific commercial tests. Higher sensitivities (>94 and >97%) and specificities (>91 and >94%) were observed for SeroHSV1 and SeroHSV2, respectively, relative to the other commercial kits. The detection of total antibodies instead of only one type of antibody (IgG or IgM) probably leads to better evaluation of the actual prevalence rates of HSV infections. Indeed, one serum sample that was found positive by SeroHSV1 and negative by the Gull HSV1 IgG was IgM positive. Due to the use of the competition technique, SeroHSV2 and SeroHSV1 might also serve as screening and confirmatory tools in large epidemiological studies for diagnosis of HSV-1 and -2 infections. Competition-based enzyme immunoassays have been successfully used previously for the identification of HSV-1 and -2 antibodies (11, 12).
Real confusion exists regarding the quality of the results obtained by the kits that are currently commercially available for the detection of HSV IgM antibodies. Many of the results obtained by the different tests cannot be confirmed by using a second test (Table 3). In an attempt to improve the current clinical diagnostic value of HSV IgM antibody testing, Savyon Diagnostics has developed the SeroHSV IgM test. We have optimized the removal of rheumatoid factor in order to eliminate IgG interference without lowering the sensitivity of the test. SeroHSV IgM is highly specific (>92%) compared to three different commercial kits (Gull, Virotech, and Dade Behring). The sensitivity of SeroHSV IgM ranges between 50 and 70% relative to the tests it is compared to. The lowest correlation was found with the Diasorin IgM test.
The best agreement was observed when SeroHSV IgM was compared to the Dade Behring test: a sensitivity of 70% and a specificity of 92%. A competition assay was run in order to clarify the discrepant results, and it clearly supports the SeroHSV IgM results rather than the Dade Behring HSV IgM test results.
SeroHSV IgG is highly sensitive (>94%) and highly specific (>92%) compared to the available commercial kits. The best relative sensitivity and specificity was obtained with the Biokit, Dade Behring, and Savyon Diagnostics (combined SeroHSV1 and SeroHSV2) tests. Both SeroHSV IgG and the Dade Behring IgG test can be used as screening tools for the detection of HSV IgG antibodies. The intended use of the MRL immunoblot test is to distinguish HSV-1 from HSV-2 antibodies in prescreened samples. Each sample is tested in a single mode, and therefore this test is not appropriate for mass screening. SeroHSV kits are ELISA based, automatable, and user friendly, and therefore they are appropriate for screening.
We believe that the major advantage in specific typing of HSVs lies in improved diagnosis of HSV-2 and the consequent prevention of the spread of HSV infection to neonates and sexual partners. The majority of the population may be asymptomatic and may show signs of carrying the virus, which may lead to active maternal infection during pregnancy and damage to the newborn at the time of delivery (14). Although specific antiviral therapy is not yet used in pregnancies, it will not be long before such therapy is advocated, mainly for HSV-2-seropositive women. HSV-1 infection through sexual contact has increased significantly during the past few years. The prognosis of HSV-1 infection is much better than that of HSV-2; therefore, it is recommended that genital herpes be detected by a specific test for HSV antibodies and confirmed by culture and typing of the isolates. Indications for using nonspecific HSV-1 and -2 IgG and IgM tests include conditions such as rashes in immunocompromised patients, suspected HSV encephalitis, and other severe or complicated HSV infections.
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ACKNOWLEDGMENTS |
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We thank our colleagues; M. H. Maayan, C. Steuerman, and A. Kilman of the Clinical Microbiology Laboratory, Sapir Medical Center (Meir Hospital), Kfar Sava, Israel, and J. Ossman of the Serology Laboratory, Central Laboratory for Haifa and Western Galilee, Clalit Health Services, Nesher, Israel, for cooperation and assistance in collecting and characterizing the samples used in this study.
The death of Abraham Morag, a senior expert in infectious disease research and one of the pioneers of HIV research in Israel, is a great loss to his family, friends, and the scientific community.
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FOOTNOTES |
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* Corresponding author. Mailing address: Savyon Diagnostics Ltd., Kiryat Minrav, 3 Habosem St., Ashdod 77610, Israel. Phone: 972-8-8562920. Fax: 972-8-8563258. E-mail: savyon_d{at}netvision.net.il.
Deceased.
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 904-908, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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43: 4215-4217
[Abstract] [Full Text]
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