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Clinical and Diagnostic Laboratory Immunology, Jan 1994, 68-70, Vol 1, No. 1
Copyright © 1994 by the American Society for Microbiology. All rights reserved.

Detection of beta-2 transferrin in otorrhea and rhinorrhea in a routine clinical laboratory setting

DE Normansell, EK Stacy, CF Booker and TZ Butler
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.

A simple, straightforward, and rapid method for the detection of beta-2 transferrin in otorrhea and rhinorrhea that can be used in a routine clinical laboratory is described. The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipment, followed by pressure transfer to a nitrocellulose membrane and then incubation with enzyme-labeled antitransferrin antibody and substrate. The procedure was fast (3.5 h) and sensitive (detected as little as 1 microgram/ml) and required only 3 microliters of fluid. Beta-2 transferrin was detected in cerebrospinal fluid diluted up to eightfold. No special training or expertise was needed, and all equipment and procedures used are commonly available in a routine clinical laboratory.

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