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Clinical and Diagnostic Laboratory Immunology, January 2003, p. 154-160, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.154-160.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Vesicular Stomatitis Virus Pseudotypes Bearing Hantaan or Seoul Virus Envelope Proteins in a Rapid and Safe Neutralization Test

Michiko Ogino,1 Hideki Ebihara,1 Byoung-Hee Lee,1 Koichi Araki,2 Åke Lundkvist,3 Yoshihiro Kawaoka,4 Kumiko Yoshimatsu,1 and Jiro Arikawa1*

Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine, Sapporo 060-8638,1 Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan,2 Swedish Institute for Infectious Disease Control and Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden,3 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin4

Received 5 April 2002/ Returned for modification 13 August 2002/ Accepted 12 October 2002

A vesicular stomatitis virus (VSV) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. The recombinant VSV, in which the enveloped protein gene (G) was replaced by the green fluorescent protein gene and complemented with G protein expressed in trans (VSV{Delta}G*G), was kindly provided by M. A. Whitt. 293T cells were transfected with plasmids for the expression of envelope glycoproteins (G1 and G2) of HTNV or SEOV and were then infected with VSV{Delta}G*G. Pseudotype VSV with the Hantaan (VSV{Delta}G*-HTN) or Seoul (VSV{Delta}G*-SEO) envelope glycoproteins were harvested from the culture supernatant. The number of infectious units (IU) of the pseudotype VSVs ranged from 105 to 106/ml. The infectivity of VSV{Delta}G*-HTN and VSV{Delta}G*-SEO was neutralized with monoclonal antibodies, immune rabbit sera, and sera from patients with hemorrhagic fever with renal syndrome, and the neutralizing titers were similar to those obtained with native hantaviruses. These results show that VSV{Delta}G*-HTN and -SEO can be used as a rapid, specific, and safe neutralization test for detecting hantavirus-neutralizing antibodies as an effective substitute for the use of native hantaviruses. Furthermore, the IU of VSV{Delta}G*-HTN and -SEO did not decrease by more than 10-fold when stored at 4°C for up to 30 days. The stability of the pseudotype viruses allows distribution of the material to remote areas by using conventional cooling boxes for use as a diagnostic reagent.

* Corresponding author. Mailing address: Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine, Kita-ku, Kita-15, Nishi-7, Sapporo 060-8638, Japan. Phone: 81-11-706-6905. Fax: 81-11-706-7879. E-mail: j_arika{at}med.hokudai.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 154-160, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.154-160.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Hanika, A., Larisch, B., Steinmann, E., Schwegmann-Wessels, C., Herrler, G., Zimmer, G. (2005). Use of influenza C virus glycoprotein HEF for generation of vesicular stomatitis virus pseudotypes. J. Gen. Virol. 86: 1455-1465 [Abstract] [Full Text]  
  • Ogino, M., Yoshimatsu, K., Ebihara, H., Araki, K., Lee, B.-H., Okumura, M., Arikawa, J. (2004). Cell Fusion Activities of Hantaan Virus Envelope Glycoproteins. J. Virol. 78: 10776-10782 [Abstract] [Full Text]  


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