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Clinical and Diagnostic Laboratory Immunology, January 2003, p. 53-58, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.53-58.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Alterations in T-Cell Receptor Vß Repertoire of CD4 and CD8 T Lymphocytes in Human Immunodeficiency Virus-Infected Children

Monica Kharbanda, Thomas W. McCloskey, Rajendra Pahwa, Mei Sun, and Savita Pahwa*

Immunology and Inflammation Center, North Shore LIJ Research Institute, North Shore LIJ Health System, New York University School of Medicine, Manhasset, New York 11030

Received 8 January 2002/ Returned for modification 15 March 2002/ Accepted 7 October 2002

Perturbations in the T-cell receptor (TCR) Vß repertoire were assessed in the CD4 and CD8 T lymphocytes of human immunodeficiency virus (HIV)-infected children who were receiving therapy during the chronic phase of infection by flow cytometry (FC) and PCR analysis. By FC, representation of 21 TCR Vß subfamilies was assessed for an increased or decreased percentage in CD4 and CD8 T cells, and by PCR, 22 TCR Vß subfamilies of CD4 and CD8 T cells were analyzed by CDR3 spectratyping for perturbations and reduction in the number of peaks, loss of Gaussian distribution, or clonal dominance. The majority of the TCR Vß subfamilies were examined by both methods and assessed for deviation from the norm by comparison with cord blood samples. The CD8-T-lymphocyte population exhibited more perturbations than the CD4 subset, and clonal dominance was present exclusively in CD8 T cells. Of the 55 total CD8-TCR Vß families classified with clonal dominance by CDR3 spectratyping, only 18 of these exhibited increased expression by FC. Patients with high numbers of CD8-TCR Vß families with decreased percentages had reduced percentages of total CD4 T cells. Increases in the number of CD4-TCR Vß families with increased percentages showed a positive correlation with skewing. Overall, changes from normal were often discordant between the two methods. This study suggests that the assessment of HIV-induced alterations in TCR Vß families at cellular and molecular levels yields different information and that our understanding of the immune response to HIV is still evolving.

* Corresponding author: Savita Pahwa, M.D., North Shore LIJ Research Institute, 350 Community Dr., Manhasset, NY 11030. Phone: (516) 562-4641. Fax: (516) 562-2866. E-mail: spahwa{at}nshs.edu.

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 53-58, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.53-58.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fernandes, S., Chavan, S., Chitnis, V., Kohn, N., Pahwa, S. (2005). Simplified Fluorescent Multiplex PCR Method for Evaluation of the T-Cell Receptor V{beta}-Chain Repertoire. CVI 12: 477-483 [Abstract] [Full Text]  


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