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Clinical and Diagnostic Laboratory Immunology, March 2003, p. 229-232, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.229-232.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens

Yee-Hsuan Chiou,1 Chung-Yee Yuo,2 Lin-Yu Wang,3 and Shiao-ping Huang3*

Department of Pediatrics, Kaohsiung Veterans General Hospital and National Yang-Ming University,1 Department of Biology, Kaohsiung Medical University,2 Department of Medical Technology, Fooyin University, Ta-Liao Hsiang, Kaohsiung Hsien, Taiwan, Republic of China3

Received 29 August 2002/ Returned for modification 4 September 2002/ Accepted 5 December 2002

The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.

* Corresponding author. Mailing address: Department of Medical Technology, Fooyin University, Ta-Liao Hsiang, Kaohsiung Hsien, 831, Taiwan, Republic of China. Phone: 886-7-782-7162. Fax: 886-7-782-7162. E-mail: sphuang{at}mail.fy.edu.tw.

Clinical and Diagnostic Laboratory Immunology, March 2003, p. 229-232, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.229-232.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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