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Clinical and Diagnostic Laboratory Immunology, March 2003, p. 323-328, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.323-328.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Assessment of Thymic Activity in Human Immunodeficiency Virus-Negative and -Positive Adolescents by Real-Time PCR Quantitation of T-Cell Receptor Rearrangement Excision Circles

Thao Pham,¹ Marvin Belzer,² Joseph A. Church,² Christina Kitchen,¹ Craig M. Wilson,³ Steven D. Douglas,⁴ Yongzhi Geng,¹ Monica Silva,¹ Richard M. Mitchell,⁵ and Paul Krogstad^1*

David Geffen School of Medicine, University of California, Los Angeles,¹ Childrens Hospital Los Angeles and the Keck School of Medicine at University of Southern California, Los Angeles, California,² University of Alabama at Birmingham School of Medicine, Birmingham, Alabama,³ University of Pennsylvania School of Medicine and Children's Hospital of Philadelphia, Philadelphia, Pennsylvania,⁴ Westat, Inc., Rockville, Maryland⁵

Received 27 August 2002/ Returned for modification 1 October 2002/ Accepted 25 November 2002

Circular DNA molecules known as T-cell receptor rearrangement excision circles (TREC) arise during T-cell development and are present in cells that have recently emigrated from the thymus. In cross-sectional studies, the number of peripheral blood lymphocytes bearing TREC decreases with age, consistent with an anatomically demonstrated loss of thymic epithelial tissue. TREC numbers increase following hematopoietic stem cell transplantation and during therapy for human immunodeficiency virus (HIV) infection. Quantitation of TREC has therefore been proposed as a parameter of thymic activity. In this study, we used real-time PCR to quantify TREC in peripheral blood samples obtained longitudinally from HIV-seronegative adolescents. TREC values in peripheral blood T cells were very stable throughout adolescence, once thought to be a time of rapid involution of the thymus. In addition, in a cross-sectional analysis, we examined TREC values in a cohort of HIV-positive adolescents and found evidence of ongoing thymopoiesis in perinatally infected individuals, despite lifelong infection. These data demonstrate the utility of TREC assessment in adolescents and that HIV infection does not uniformly result in accelerated thymic involution in childhood.

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* Corresponding author. Mailing address: University of California, Los Angeles, Division of Pediatric Infectious Diseases, 22-442 MDCC, 10833 Le Conte Avenue, Los Angeles, CA 90095. Phone: (310) 825-5269. Fax: (310) 206-5529. E-mail: pkrogstad{at}mednet.ucla.edu.

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Clinical and Diagnostic Laboratory Immunology, March 2003, p. 323-328, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.323-328.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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