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Clinical and Diagnostic Laboratory Immunology, May 2003, p. 357-361, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.357-361.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effects of Sample Collection and Storage Methods on Antipneumococcal Immunoglobulin A in Saliva

A. Nurkka,1* J. Obiero,2 H. Käyhty,1 and J. A. G. Scott2,3

National Public Health Institute, Department of Vaccines, Helsinki, Finland,1 Wellcome Trust/Kenya Medical Research Institute, Centre for Geographic Medicine Research—Coast, Kilifi, Kenya,2 Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom3

Received 11 July 2002/ Returned for modification 22 October 2002/ Accepted 27 January 2003

Saliva contains components of both the mucosal and systemic immune systems. Variable flow rates, immunoglobulin proteases, and variation in collection and storage methods all introduce differences in the estimated concentrations of antibodies. We evaluated the effect of four collection methods and three storage protocols on the concentrations of immunoglobulin A (IgA) antibodies to pneumococcal capsular antigens 1, 5, 6B, and 14 and to pneumococcal surface adhesin A (PsaA) in saliva. Specimens were collected from 30 healthy Kenyan adults by collecting drool, by pipette suction, and with two commercial kits, OraSure and Oracol. Aliquots from each specimen were snap-frozen with glycerol in liquid nitrogen or stored for 4 to 8 h at +4°C either with or without the addition of protease enzyme inhibitors prior to storage at -70°C. Anticapsular IgA concentrations were not significantly different with different collection methods, but snap-freezing the specimens in liquid nitrogen led to concentrations 41 to 47% higher than those of specimens stored by the other methods (P < 0.0005).

* Corresponding author. Mailing address: National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358 9 4744 8301. Fax: 368 9 4744 8599. E-mail: Anu.Nurkka{at}ktl.fi.

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 357-361, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.357-361.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Booth, C. K, Dwyer, D. B, Pacque, P. F, Ball, M. J (2009). Measurement of immunoglobulin A in saliva by particle-enhanced nephelometric immunoassay: sample collection, limits of quantitation, precision, stability and reference range. Ann Clin Biochem 46: 401-406 [Abstract] [Full Text]  


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