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Clinical and Diagnostic Laboratory Immunology, July 2003, p. 680-685, Vol. 10, No. 4
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.4.680-685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Production and Characterization of a Human Recombinant Monoclonal Fab Fragment Specific for Influenza A Viruses

Alessandra Desogus,1,2 Roberto Burioni,3 Angela Ingianni,1 Francesca Bugli,4 Raffaello Pompei,1* and Giovanni Fadda4

Sezione di Microbiologia Applicata, Dipartimento di Scienze e Tecnologie Biomediche, Università di Cagliari,1 Biotecne, Cagliari,2 Istituto di Microbiologia e Scienze Biomediche, Università di Ancona, Ancona,3 Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome, Italy4

Received 27 September 2002/ Returned for modification 20 December 2002/ Accepted 25 February 2003

A human recombinant monoclonal Fab fragment that specifically recognizes all the influenza A virus strains tested was produced in transformed Escherichia coli using the phage display technique. No strain of influenza B virus reacted with it. It was purified after four cycles of panning and by a single passage through an immunoaffinity column. About 1 mg of pure monoclonal antibody was obtained from 1 liter of culture medium in 3 working days. The Fab fragment reacted with a viral 27-kDa protein, which could reasonably be a matrix protein. Indirect immunofluorescence tests performed on virus-infected MDCK cells showed that this Fab fragment was at least equally efficient as other commercial monoclonal antibody-based systems in detecting influenza A viral infections. The potential advantages of human recombinant Fabs on murine monoclonal antibodies are discussed.

* Corresponding author. Mailing address: Dep. Scienze e Tecnologie Biomediche, Sez. Microbiologia, Via Porcell 4, 09124 Cagliari, Italy. Phone: 070 675 8483. Fax: 070 675 8482. E-mail: rpompei{at}unica.it.

Clinical and Diagnostic Laboratory Immunology, July 2003, p. 680-685, Vol. 10, No. 4
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.4.680-685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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