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Clinical and Diagnostic Laboratory Immunology, September 2003, p. 787-792, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.787-792.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Probiotic Bacteria on Induction and Maintenance of Oral Tolerance to ß-Lactoglobulin in Gnotobiotic Mice

Guénolée Prioult,1,2 Ismail Fliss,1* and Sophie Pecquet1,2

Dairy Research Centre STELA, Département des Sciences des Aliments et de Nutrition, Université Laval, Québec, Quebec G1K 7P4, Canada,1 Food Immunology Group, Nestec SA, Nestlé Research Center Lausanne, Vers-Chez-les-Blanc, CH-1000 Lausanne 26, Switzerland2

Received 7 February 2003/ Returned for modification 22 April 2003/ Accepted 22 May 2003

In this study, the effect of Lactobacillus paracasei (NCC 2461), Lactobacillus johnsonii (NCC 533) and Bifidobacterium lactis Bb12 (NCC 362) on the induction and maintenance of oral tolerance to bovine ß-lactoglobulin (BLG) was investigated in mice. Germfree mice were monocolonized with one of the three strains before oral administration of whey protein to induce tolerance. Mice were then injected with BLG and sacrificed 28 or 50 days after whey protein feeding for humoral and cellular response measurement. Conventional and germfree mice were used as controls. Both humoral and cellular responses were better suppressed in conventional mice than in germfree and monoassociated mice throughout the experiment and better suppressed in L. paracasei-associated mice than in mice colonized with B. lactis or L. johnsonii. The latter two mono-associations suppressed humoral responses only partially and cellular responses not at all. This study provides evidence that probiotics modulate the oral tolerance response to BLG in mice. The mono-colonization effect is strain-dependant, the best result having been obtained with L. paracasei.

* Corresponding author. Mailing address: Centre STELA, Pavillon Paul Comtois, Université Laval, Québec, Quebec G1K 7P4, Canada. Phone: (418) 656-2131, x6825. Fax: (418) 656-3353. E-mail: ismail.fliss{at}aln.ulaval.ca.

Clinical and Diagnostic Laboratory Immunology, September 2003, p. 787-792, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.787-792.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Prioult, G., Pecquet, S., Fliss, I. (2004). Stimulation of Interleukin-10 Production by Acidic {beta}-Lactoglobulin-Derived Peptides Hydrolyzed with Lactobacillus paracasei NCC2461 Peptidases. CVI 11: 266-271 [Abstract] [Full Text]  


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