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Clinical and Diagnostic Laboratory Immunology, September 2003, p. 967-968, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.967-968.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Modified Direct Agglutination Test for Simplified Serologic Diagnosis of Leishmaniasis

P. Gómez-Ochoa,1* J. A. Castillo,1 J. Lucientes,1 M. Gascón,1 J. J. Zarate,1 J. I. Arbea,1 V. Larraga,2 and C. Rodriguez1

Departamento de Patología Animal, Facultad de Veterinaria de Zaragoza, Zaragoza,1 Centro de Investigaciones Biológicas (CSIC), Madrid, Spain2

Received 8 July 2002/ Returned for modification 15 October 2002/ Accepted 21 March 2003

Human leishmaniasis is a severe health problem in many countries around the world. Hence, a cheap, reliable, and accurate diagnostic test is required to fight this disease. Perhaps the direct agglutination test (DAT) meets these criteria, but antigen elaboration involves many difficulties. We have developed a new antigen elaboration method, the EasyDAT method, that avoids the problems associated with the DAT. In this study, we compared the traditional DAT antigen method with our EasyDAT antigen method by using canine sera. The sensitivities (100%) and specificities (98.7%) were the same for both methods; we therefore concluded that the EasyDAT Leishmania antigen method simplifies serologic diagnosis, making this method easier and cheaper to use.

* Corresponding author. Mailing address: Departamento de Patología Animal, Facultad de Veterinaria de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. Phone: 34 976 76 1633. Fax: 34 976 76 76 1612. E-mail: pablogomezochoa{at}yahoo.es.

Clinical and Diagnostic Laboratory Immunology, September 2003, p. 967-968, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.967-968.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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