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Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1109-1116, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1109-1116.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evolution of Two Amino Acid Positions Governing Broad Neutralization Resistance in a Strain of Feline Immunodeficiency Virus over 7 Years of Persistence in Cats

Mauro Pistello, Donatella Matteucci, Simone Giannecchini, Francesca Bonci, Olimpia Sichi, Silvano Presciuttini, and Mauro Bendinelli*

Retrovirus Center and Virology Section, Department of Experimental Pathology, University of Pisa, Pisa, Italy

Received 6 June 2003/ Returned for modification 12 August 2003/ Accepted 26 August 2003

Fresh isolates of lentiviruses are characterized by an outstanding resistance to antibody-mediated neutralization. By investigating the changes that occurred in a neutralization-sensitive tissue culture-adapted strain of feline immunodeficiency virus after it was reinoculated into cats, a previous study had identified two amino acid positions of the surface glycoprotein (residues 481 and 557) which govern broad neutralization resistance (BNR) in this virus. By extending the follow-up of six independently evolving in vivo variants of such virus for up to 92 months, we now show that the changes at the two BNR-governing positions not only were remarkably stereotyped but also became fixed in an ordered sequential fashion with the duration of in vivo infection. In one variant, the two positions were also seen to slowly alternate at determining BNR. Evidence that evolution at the BNR-governing positions was accompanied, and possibly driven, by changes in the antigenic makeup of the viral surface brought about by the mutations at such positions is also presented.

* Corresponding author. Mailing address: Dipartimento di Patologia Sperimentale, Università di Pisa, Via San Zeno 37, I-56127 Pisa, Italy. Phone: 39 050 2213 641. Fax: 39 050 2213 639. E-mail: bendinelli{at}biomed.unipi.it.

Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1109-1116, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1109-1116.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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