This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rajavelu, P.
Right arrow Articles by Das, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rajavelu, P.
Right arrow Articles by Das, S. D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1149-1152, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1149-1152.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cell-Mediated Immune Responses of Healthy Laboratory Volunteers to Sonicate Antigens Prepared from the Most Prevalent Strains of Mycobacterium tuberculosis from South India Harboring a Single Copy of IS6110

Priya Rajavelu and Sulochana D. Das*

Tuberculosis Research Centre (ICMR), Chennai, India

Received 9 May 2003/ Returned for modification 27 June 2003/ Accepted 25 July 2003

Our restriction fragment length polymorphism (RFLP) studies have shown that the most prevalent (40%) strains of Mycobacterium tuberculosis from South India contain a single copy of the IS6110 insertion sequence and are of importance in studying virulence and immunity. Sonicate antigens from seven such strains were used to study in vitro T-cell proliferation and gamma interferon (IFN-{gamma}) and interleukin-12 (IL-12) secretion as markers of protective immunity in 25 healthy subjects positive for purified protein derivative (PPD). The standard PPD and heat-killed H37Rv antigens induced the maximum levels of T-cell proliferation and IFN-{gamma} secretion but low levels of IL-12. All sonicate antigens induced T-cell proliferation and IFN-{gamma} secretion with strong positive correlation. Our results suggest that sonicate antigens from the most prevalent and recent strains of M. tuberculosis from clinical isolates have the potential to induce T-cell activation and may allow newer and specific antigens to be further characterized for diagnosis and vaccine development.

* Corresponding author. Mailing address: Tuberculosis Research Centre (ICMR), Mayor V. R. Ramanathan Rd., Chetput, Chennai 600 031, India. Phone: 91-044-28362435. Fax: 91-044-28362528. E-mail: sulochanadas{at}rediffmail.com.

Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1149-1152, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1149-1152.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»