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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 131-136, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.131-136.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection and Differentiation of Anti-Helicobacter pylori Antibodies by Flow Microparticle Immunofluorescence Assay

F. Bühling,1* G. Koch,1,2 T. Wex,3 A. Heimburg,1,2 M. Vieth,4 A. Leodolter,3 A. Roessner,4 S. Ansorge,2 and P. Malfertheiner3

Institute of Immunology,1 Department of Gastroenterology,3 Department of Pathology, Otto-von-Guericke University Magdeburg,4 Institute of Medical Technology Magdeburg (IMTM) GmbH, 39120 Magdeburg, Germany2

Received 1 August 2003/ Returned for modification 26 September 2003/ Accepted 7 November 2003

Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are currently detected by immunoblot analysis. At present, the immunodiagnosis of an H. pylori infection includes two separate methods: ELISA and immunoblot analysis. Here, we report the development and evaluation of a new rapid flow microparticle immunofluorescence assay (FMIA) for detection of anti-H. pylori antibodies in human serum. The assay allows rapid qualitative and quantitative detection of anti-H. pylori antibodies by using crude antigen preparations as well as single recombinant antigens (urease A, urease B, CagA, and alkylhydroxy peroxide reductase) in the same sample with one measurement, and thus it combines the advantages of enzyme immunoassay and Western blot analysis. Seventy-five patient samples were analyzed by FMIA, ELISA, and Western blotting with respect to their immunoreactivity against crude H. pylori extracts and individual H. pylori antigens. Statistical analyses revealed an overall similarity of more than 90% among the results for FMIA, ELISA, and Western blot. Therefore, we conclude that FMIA is a powerful and time- and cost-saving assay system for the detection of antimicrobial antibodies, with higher sensitivity and a larger measurement range than ELISA.

* Corresponding author. Mailing address: Institute of Immunology, Otto-von-Guericke University Magdeburg, Leipziger-Str. 44, 39120 Magdeburg, Germany. Phone: 49 391 6713311. Fax: 49 391 67190466. E-mail: frank.buehling{at}medizin.uni-magdeburg.de.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 131-136, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.131-136.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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