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Clinical and Diagnostic Laboratory Immunology, 09 1996, 608-610, Vol 3, No. 5
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: production, characterization, and immunodiagnostic application

F Qadri, MG Mohi, A Chowdhury, K Alam, T Azim, C Sears, RB Sack and MJ Albert
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.

A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the "gold standard," the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

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