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Clinical and Diagnostic Laboratory Immunology, Jan 1997, 4-10, Vol 4, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

A semiquantitative assay for CD8+ T-cell-mediated suppression of human immunodeficiency virus type 1 infection

Y Chen, C Rinaldo and P Gupta
Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health and School of Medicine, Pennsylvania 15261, USA.

A reproducible, semiquantitative assay was developed to measure the level of the anti-human immunodeficiency virus type 1 (anti-HIV-1) suppressive activity of CD8+ T cells. The assay had a wide dynamic range and could be applied to a relatively small number of fresh and cryopreserved peripheral blood mononuclear cells and purified CD8+ T cells. The suppressive activity was not due to cytolytic activity and was not major histocompatibility complex class I restricted. Suppression of HIV-1 infection by CD8+ T cells was consistently demonstrable with both endogenously infected autologous CD4+ T cells and exogenously infected allogeneic CD4+ T cells. This assay can be used to monitor the level of antiviral activity of CD8+ T cells in a retrospective and prospective manner in studies of the natural history of HIV-1 infection and of subjects receiving anti-HIV-1 therapy and vaccines.


This article has been cited by other articles:

  • Geiben-Lynn, R., Kursar, M., Brown, N. V., Kerr, E. L., Luster, A. D., Walker, B. D. (2001). Noncytolytic Inhibition of X4 Virus by Bulk CD8+ Cells from Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Persons and HIV-1-Specific Cytotoxic T Lymphocytes Is Not Mediated by {beta}-Chemokines. J. Virol. 75: 8306-8316 [Abstract] [Full Text]  


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