This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kook, H. Articles by Trigg, M. Search for Related Content PubMed PubMed Citation Articles by Kook, H. Articles by Trigg, M.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 01 1997, 96-103, Vol 4, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Reconstruction of the immune system after unrelated or partially matched T-cell-depleted bone marrow transplantation in children: functional analyses of lymphocytes and correlation with immunophenotypic recovery following transplantation

H Kook, F Goldman, R Giller, N Goeken, C Peters, M Comito, S Rumelhart, M Holida, N Lee and M Trigg
Department of Pediatrics, Chonnam University, Kwangju, Korea.

Reconstitution of the immune system following T-cell-depleted bone marrow transplantation (BMT) in children has yet to be fully elucidated. Thus, we prospectively studied the recovery of immune function in 64 children who underwent T-lymphocyte-depleted marrow transplants using either matched family member donors or matched unrelated donors. We measured in vitro posttransplantation proliferative responses to phytohemagglutinin (PHA), concanavalin A, pokeweed mitogen, and Candida albicans antigen and assessed unidirectional allogeneic mixed-lymphocyte culture (MLC) responses at various times. A total of 129 healthy individuals served as normal controls for these assays. Responses to T-cell mitogens normalized within 12 months posttransplantation, while MLC responses normalized by 9 months. The presence of graft-versus-host disease (grade II or greater) and cytomegalovirus infection was associated with delays in immune function recovery. Importantly, immune function recovery correlated temporally with a rise in peripheral lymphocyte count. In contrast, the CD4/CD8 ratio was not predictive of immune recovery. Knowledge of immune function recovery may guide clinicians in devising strategies to minimize the risk of infection post-BMT.

This article has been cited by other articles:

• Trigg, M. E. (2004). Hematopoietic Stem Cells. Pediatrics 113: 1051-1057 [Abstract] [Full Text]  
• Heitger, A., Winklehner, P., Obexer, P., Eder, J., Zelle-Rieser, C., Kropshofer, G., Thurnher, M., Holter, W. (2002). Defective T-helper cell function after T-cell-depleting therapy affecting naive and memory populations. Blood 99: 4053-4062 [Abstract] [Full Text]  
• Thomson, B. G., Robertson, K. A., Gowan, D., Heilman, D., Broxmeyer, H. E., Emanuel, D., Kotylo, P., Brahmi, Z., Smith, F. O. (2000). Analysis of engraftment, graft-versus-host disease, and immune recovery following unrelated donor cord blood transplantation. Blood 96: 2703-2711 [Abstract] [Full Text]