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Clinical and Diagnostic Laboratory Immunology, 11 1997, 783-786, Vol 4, No. 6
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Clinical field site evaluation of the FACSCount for absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell count determinations in Thailand

NL Young, P Ponglertnapakorn, N Shaffer, K Srisak, T Chaowanachan, V On-Thern, C Kittinunvorakoon, A Bunwattanakul, S Suksaweang, V Pobkeeree, J Punnotok and TD Mastro
HIV/AIDS Collaboration and Department of Communicable Disease Control, Central Chest Hospital, Nonthaburi, Thailand. NLY0@cdc.gov

The FACSCount flow cytometer absolute CD3+, CD3+ CD4+, and CD3+ CD8+ cell counts measured at a field site hospital laboratory in Thailand were compared to FACScan absolute counts obtained at a nearby research laboratory. Correlation coefficients for 208 samples were > or = 0.95. The FACSCount was accurate, and it was easier and less expensive to operate than the FACScan. Additionally, the FACScan-generated lymphocyte percentage value was accurate for use with the FACScan SimulSET software.


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