This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Linke, M. J.
Right arrow Articles by Walzer, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Linke, M. J.
Right arrow Articles by Walzer, P. D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 50-57, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression, Structure, and Location of Epitopes of the Major Surface Glycoprotein of Pneumocystis carinii f. sp. carinii

Michael J. Linke,1,2,* Susan M. Sunkin,3 Ryan P. Andrews,1 James R. Stringer,3 and Peter D. Walzer1,2

Cincinnati Veterans Affairs Medical Center,1 Department of Internal Medicine, University of Cincinnati College of Medicine,2 and Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine,3 Cincinnati, Ohio

Received 29 May 1997/Returned for modification 31 July 1997/Accepted 2 October 1997

The major surface glycoprotein (MSG) of Pneumocystis carinii f. sp. carinii consists of a heterogeneous family of proteins that are encoded by approximately 100 unique genes. A genomic expression library was screened with a panel of MSG-specific monoclonal antibodies (MAbs) to identify conserved and rare epitopes. All of the antibodies reacted with epitopes that are encoded within the 5' end of MSG. The results from the expression screening identified antibodies that recognize highly conserved, moderately conserved, and rare epitopes. Four MAbs (MAbs RA-F1, RA-E7, RA-G10, and RB-E3) reacted with a maltose binding protein-MSG-B fusion protein (MBPMSG-B41-1065) by immunoblotting and enzyme-linked immunosorbent assay. Three of the MAbs (MAbs RA-F1, RA-G10, and RA-E7) reacted with the same continuous epitope that was localized to amino acids 278 to 290 of MSG-B. Comparison of the sequence of the RA-F1-, RA-G10-, and RA-E7-reactive epitope to the deduced amino acid sequences of multiple MSGs demonstrated that it is highly conserved. The reactivity of RB-E3 with MSG-B was shown to be dependent on amino acids 184 to 192, which may comprise a portion of a discontinuous epitope.

* Corresponding author. Mailing address: VA Medical Center, 3200 Vine St., Cincinnati, OH 45220. Phone: (513) 861-3100, extension 4423. Fax: (513) 475-6415. E-mail: mjl19{at}juno.com.

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 50-57, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cushion, M. T., Keely, S. P., Stringer, J. R. (2004). Molecular and phenotypic description of Pneumocystis wakefieldiae sp. nov., a new species in rats. Mycologia 96: 429-438 [Abstract] [Full Text]  
  • Schaffzin, J. K., Stringer, J. R. (2004). Expression of the Pneumocystis carinii major surface glycoprotein epitope is correlated with linkage of the cognate gene to the upstream conserved sequence locus. Microbiology 150: 677-686 [Abstract] [Full Text]  
  • Walzer, P. D. (1999). Immunological Features of Pneumocystis carinii Infection in Humans. CVI 6: 149-155 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»