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Clinical and Diagnostic Laboratory Immunology, January 1998, p. 78-81, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytokine Production in Cell Culture by Peripheral Blood Mononuclear Cells from Immunocompetent Hosts

Rohit K. Katial,1,* Doris Sachanandani,2 Carolyn Pinney,2 and Michael M. Lieberman2,dagger

Department of Allergy and Immunology, Walter Reed Army Medical Center, Washington, D.C.,1 and Department of Clinical Investigation, Fitzsimons Army Medical Center, Aurora, Colorado2

Received 11 June 1997/Returned for modification 30 July 1997/Accepted 21 October 1997

The production of interleukin 2 (IL-2) gamma interferon, IL-4, tumor necrosis factor alpha (TNF-alpha ), TNF-beta , IL-5, and IL-10 in vitro by peripheral blood mononuclear cells cultured from healthy immunocompetent subjects after mitogen stimulation was determined. The mitogens used were concanavalin A, phytohemagglutinin, pokeweed mitogen, and Staphylococcus aureus Cowen. The results obtained provide a normal range for the production of these cytokines under specified conditions in vitro.

* Corresponding author. Mailing address: Department of Allergy and Immunology, Walter Reed Army Medical Center, Washington, DC 20307. Phone: (202) 782-8085. Fax: (202) 782-7093. E-mail: RKatial{at}POL.NET.

dagger Present address: Department of Chemistry, University of Hawaii at Manoa, Honolulu, HI 96822.

Clinical and Diagnostic Laboratory Immunology, January 1998, p. 78-81, Vol. 5, No. 1
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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