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Clinical and Diagnostic Laboratory Immunology, March 1998, p. 160-170, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diversity of Hemagglutination Phenotypes among P-Fimbriated Wild-Type Strains of Escherichia coli in Relation to papG Allele Repertoire

James R. Johnson,* Jennifer J. Brown, and Parvia Ahmed

Medicine Service, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota

Received 16 June 1997/Returned for modification 11 November 1997/Accepted 5 December 1997

Data regarding the hemagglutination (HA) patterns of the three variants (classes I, II, and III) of the Escherichia coli adhesin PapG are conflicting. These HA patterns usually have been assessed for each papG allele separately with recombinant strains in slide HA assays. We rigorously evaluated an alternative microtiter tray HA assay and then used it to assess the HA of four erythrocyte types (human A1P1 and OP1, rabbit, and sheep erythrocytes) by multiple wild-type E. coli strains representing the four naturally occurring combinations of the papG alleles, i.e., class I plus III, class III only, class II plus III, and class II only. The microtiter tray HA assay displayed significantly better reproducibility of intraobserver (83%) and interobserver (86%) results than did slide HA assays (39 and 73%, respectively). Novel findings from the study of 32 wild-type P-fimbriated strains included reproducible determinations of phenotypic diversity among different papG categories, among strains within each papG category, and from day to day for individual strains. There was also substantial overlap of phenotypes between papG categories I plus III and III only and between II plus III and II only. A class III papG recombinant strain's HA pattern differed significantly from that of the wild-type class III strains. These data demonstrate that HA phenotypes of wild-type P-fimbriated E. coli strains can be reproducibly assessed by a microtiter HA assay and that they correspond broadly to papG genotype but in a more complex and varied fashion than previously recognized.

* Corresponding author. Mailing address: Infectious Disease (111F), VA Medical Center, One Veterans Dr., Minneapolis, MN 55417. Phone: (612) 725-2000, ext. 4185. Fax: (612) 725-2273. E-mail: johns007{at}maroon.tc.umn.edu.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 160-170, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Johnson, J. R., Stell, A. L., Kaster, N., Fasching, C., O'Bryan, T. T. (2001). Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG. Infect. Immun. 69: 2318-2327 [Abstract] [Full Text]  
  • Johnson, J. R., Stell, A. L., Delavari, P. (2001). Canine Feces as a Reservoir of Extraintestinal Pathogenic Escherichia coli. Infect. Immun. 69: 1306-1314 [Abstract] [Full Text]  
  • Johnson, J. R., O'Bryan, T. T., Low, D. A., Ling, G., Delavari, P., Fasching, C., Russo, T. A., Carlino, U., Stell, A. L. (2000). Evidence of Commonality between Canine and Human Extraintestinal Pathogenic Escherichia coli Strains That Express papG Allele III. Infect. Immun. 68: 3327-3336 [Abstract] [Full Text]  
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  • Johnson, J. R. (1998). papG Alleles among Escherichia coli Strains Causing Urosepsis: Associations with Other Bacterial Characteristics and Host Compromise. Infect. Immun. 66: 4568-4571 [Abstract] [Full Text]  


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