This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schramm, W.
Right arrow Articles by Burgess-Cassler, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schramm, W.
Right arrow Articles by Burgess-Cassler, A.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 263-265, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus

Willfried Schramm,1,dagger Stephen E. Wade,1 Gustavo Barriga Angulo,2 Patricia Castillo Torres,2 and Anthony Burgess-Cassler1,*

Saliva Diagnostic Systems, Inc., Vancouver, Washington 98682-2444,1 and Clinical Laboratory, Hospital de Infectologia, Centro Medico Nacional la Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico2

Received 8 September 1997/Returned for modification 10 November 1997/Accepted 25 November 1997

We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).

* Corresponding author. Mailing address: Saliva Diagnostic Systems, Inc., 11719 NE 95 St., Vancouver, WA 98682-2444. Phone: (360) 696-4800. Fax: (360) 254-7942. E-mail: saliva1{at}pacifier.com.

dagger Present address: Selfcare, Inc., Waltham, Mass.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 263-265, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peruski, A. H., Peruski, L. F. Jr. (2003). Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents. CVI 10: 506-513 [Full Text]  
  • Joffe, A. (2001). Improving Human Immunodeficiency Virus Testing for Adolescents. Arch Pediatr Adolesc Med 155: 761-762 [Full Text]  
  • Peralta, L., Constantine, N., Griffin Deeds, B., Martin, L., Ghalib, K. (2001). Evaluation of Youth Preferences for Rapid and Innovative Human Immunodeficiency Virus Antibody Tests. Arch Pediatr Adolesc Med 155: 838-843 [Abstract] [Full Text]  
  • Schramm, W., Angulo, G. B., Torres, P. C., Burgess-Cassler, A. (1999). A Simple Saliva-Based Test for Detecting Antibodies to Human Immunodeficiency Virus. CVI 6: 577-580 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»