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Clinical and Diagnostic Laboratory Immunology, March 1998, p. 266-269, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Absolute CD4 Counts Obtained by a Three-Color Flow-Cytometric Method without the Use of a Hematology Analyzer

Thomas S. Alexander*

Department of Pathology, Summa Health System, Akron, Ohio 44309

Received 1 August 1997/Returned for modification 23 September 1997/Accepted 25 November 1997

We evaluated the Ortho TRIO-Cytoronabsolute system for determining absolute CD4 counts. The CD4 counts in our blood specimens from 100 individuals ranged from 3 to 1,962; the percent CD4 ranged from 1.3 to 62.2, respectively. The TRIO system was biased toward lower absolute counts than a combination of flow cytometry and hematology but showed no bias in percent CD4 calculations.

* Mailing address: Department of Pathology, Summa Health System, 525 E. Market St., Akron, OH 44309. Phone: (330) 375-3719. Fax: (330) 375-4874. E-mail: talexand{at}neoucom.edu.

Clinical and Diagnostic Laboratory Immunology, March 1998, p. 266-269, Vol. 5, No. 2
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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