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Clinical and Diagnostic Laboratory Immunology, May 1998, p. 313-318, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of the Humoral Immune Response to Chlamydia Outer Membrane Protein 2

Per Mygind,1 Gunna Christiansen,1 Kenneth Persson,2 and Svend Birkelund1,*

Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark,1 and Department of Virology, The University Hospital, Malmö, Sweden2

Received 20 October 1997/Returned for modification 7 January 1998/Accepted 16 February 1998

The humoral immune response to Chlamydia outer membrane protein 2 (Omp2) was studied. Omp2 is a highly genus-conserved structural protein of all Chlamydia species, containing a variable N-terminal fragment. To analyze where the immunogenic parts were localized, seven highly purified truncated fusion proteins constituting different regions of the protein were produced (Chlamydia pneumoniae-Omp2aa23-aa93, Chlamydia psittaci-Omp2aa23-aa94, and Chlamydia trachomatis-Omp2aa23-aa84, aa87-aa547, aa23-aa182, aa167-aa434, aa420-aa547). By an enzyme-linked immunosorbent assay with serologically defined patient sera, Omp2 was found to be a major immunogen of both C. pneumoniae and C. trachomatis infections (P <<  0.0001). The humoral immune responses were not confined to any particular region of the Omp2 protein, and no species-specific anti-Omp2 immunoglobulins were detected.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, 8000 Aarhus C, Denmark. Phone: 45 89 42 17 49. Fax: 45 86 19 61 28. E-mail: chlam{at}biobase.dk.

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 313-318, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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