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Clinical and Diagnostic Laboratory Immunology, May 1998, p. 335-340, Vol. 5, No. 3
Center for Interdisciplinary Research in
Immunology and Disease at UCLA and Jonsson Comprehensive Cancer
Center, UCLA School of Medicine, Los Angeles, California 90095-1747
Received 15 August 1997/Returned for modification 21 October
1997/Accepted 2 February 1998
Sequential gene expression of two type 1 cytokines (interleukin 2 [IL-2] and gamma interferon), one type 2 cytokine (IL-10), two
monokines (IL-6 and tumor necrosis factor alpha), and one cytokine
receptor (IL-2 receptor [IL-2R]) in normal human peripheral blood
mononuclear cells (PBMC) following in vitro stimulation was
investigated by reverse transcription-PCR methods. Two stimuli were
utilized: phytohemagglutinin (PHA), which acts on the CD2 molecule and
T-cell receptors, and anti-CD3 monoclonal antibody, which acts on the
CD3 molecule and on T-cell receptors. Increased expression of all
studied genes occurred between 1 and 4 hours after stimulation, except
for that of the gene encoding IL-10, which was delayed. Expression of
all but one of the genes was transient, with a maximal mRNA
accumulation at about 8 h on average. IL-2R mRNA expression was an
exception, showing a prolonged increase (72 h). The general profiles of
expression of the five cytokine genes were similar but not identical,
suggesting some shared regulatory mechanisms. When responses to four
additional stimuli (pokeweed mitogen, Candida albicans, and
IL-2 at high and low doses) were compared, similar profiles of cytokine
gene expression were found. Thus, the various stimuli caused induction
of all cytokines with quantitative, not qualitative, differences.
Altogether, the present data are useful for defining the kinetics of
gene expression for key cytokines in response to standard immune-cell
stimuli.
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cytokine Gene Expression in Normal Human
Lymphocytes in Response to Stimulation
*
Corresponding author. Mailing address: CIRID at UCLA,
UCLA School of Medicine, 10833 Le Conte Ave., Los Angeles, CA
90095-1747. Phone: (310) 825-1997. Fax: (310) 206-1318.
Present address: Medical College of Wisconsin, Department of
Pediatrics, Milwaukee, WI 53226.
Clinical and Diagnostic Laboratory Immunology, May 1998, p. 335-340, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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