This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sacchi, C. T.
Right arrow Articles by Mayer, L. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sacchi, C. T.
Right arrow Articles by Mayer, L. W.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 348-354, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Correlation between Serological and Sequencing Analyses of the PorB Outer Membrane Protein in the Neisseria meningitidis Serotyping System

Claudio T. Sacchi,1,* Ana P. S. Lemos,1 Anne M. Whitney,2 Claude A. Solari,3 Mary E. Brandt,2 Carmo E. A. Melles,1 Carl E. Frasch,4 and Leonard W. Mayer2

Bacteriology Division, Adolfo Lutz Institute, São Paulo,1 and Bacteriology Department, Fundação Oswaldo Cruz, Rio de Janeiro,3 Brazil; Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2; and Center for Biologics Evaluation and Research, Bethesda, Maryland4

Received 31 October 1997/Returned for modification 28 January 1998/Accepted 10 February 1998

The current serological typing scheme for Neisseria meningitidis is not comprehensive; a proportion of isolates are not serotypeable. DNA sequence analysis and predicted amino acid sequences were used to characterize the structures of variable-region (VR) epitopes on N. meningitidis PorB proteins (PorB VR typing). Twenty-six porB gene sequences were obtained from GenBank and aligned with 41 new sequences. Primary amino acid structures predicted from those genes were grouped into 30 VR families of related variants that displayed at least 60% similarity. We correlated VR families with monoclonal antibody (MAb) reactivities, establishing a relationship between VR families and epitope locations for 15 serotype-defining MAbs. The current panel of serotype-defining MAbs underestimates by at least 50% the PorB VR variability because reagents for several major VR families are lacking or because a number of VR variants within some families are not recognized by serotype-defining MAbs. These difficulties, also reported for serosubtyping based on the PorA protein, are shown as inconsistent results between serological and sequence analyses, leading to inaccurate strain identification and incomplete epidemiological data. The information from this study enabled the expansion of the panel of MAbs currently available for serotyping, by including MAbs of previously undetermined specificities. Use of the expanded serotype panel enabled us to improve the sensitivity of serotyping by resolving a number of formerly nonserotypeable strains. In most cases, this information can be used to predict the VR family placement of unknown PorB proteins without sequencing the entire porB gene. PorB VR typing complements serotyping, and a combination of both techniques may be used for full characterization of meningococcal strains. The present work represents the most complete and integrated data set of PorB VR sequences and MAb reactivities of serogroup B and C meningococci produced to date.

* Corresponding author. Present address: Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-2822. Fax: (404) 639-4421. E-mail: cls9{at}cdc.gov.

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 348-354, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Corless, C. E., Kaczmarski, E., Borrow, R., Guiver, M. (2008). Molecular Characterization of Neisseria meningitidis Isolates Using a Resequencing DNA Microarray. J. Mol. Diagn. 10: 265-271 [Abstract] [Full Text]  
  • Lemos, A. P. S., Brandao, A. P., Gorla, M. C. O., Paiva, M. V., Simonsen, V., Melles, C. E. A. (2006). Phenotypic characterization of Neisseria meningitidis strains isolated from invasive disease in Brazil from 1990 to 2001. J Med Microbiol 55: 751-757 [Abstract] [Full Text]  
  • Dyet, K. H., Martin, D. R. (2005). Sequence Variation in the porB Gene from B:P1.4 Meningococci Causing New Zealand's Epidemic. J. Clin. Microbiol. 43: 838-842 [Abstract] [Full Text]  
  • Lynn, F., Hobbs, M. M., Zenilman, J. M., Behets, F. M. T. F., Van Damme, K., Rasamindrakotroka, A., Bash, M. C. (2005). Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections. J. Clin. Microbiol. 43: 368-375 [Abstract] [Full Text]  
  • Law, D. K. S., Henderson, A. M., Tsang, R. S. W. (2004). DNA Sequence Analysis of the PorB Protein of Nonserotypeable Serogroup C ET-15 Meningococci Suggests a Potential Mutational Hot Spot on Their Serotype Antigens. J. Clin. Microbiol. 42: 2718-2723 [Abstract] [Full Text]  
  • Tsang, R. S. W., Tsai, C. M., Zhu, P., Ringuette, L., Lorange, M., Law, D. K. S. (2004). Phenotypic and Genetic Characterization of a Unique Variant of Serogroup C ET-15 Meningococci (with the Antigenic Formula C:2a:P1.7,1) Causing Invasive Meningococcal Disease in Quebec, Canada. J. Clin. Microbiol. 42: 1460-1465 [Abstract] [Full Text]  
  • Dyet, K. H., Simmonds, R. S., Martin, D. R. (2004). Evaluation of porB PCR-Amplicon Restriction Endonuclease Analysis as a Method To Determine porB Variable-Region Sequences in Nonserotypeable Meningococci. J. Clin. Microbiol. 42: 1731-1733 [Abstract] [Full Text]  
  • Tsang, R. S.W., Kiefer, L., Law, D. K. S., Stoltz, J., Shahin, R., Brown, S., Jamieson, F. (2003). Outbreak of Serogroup C Meningococcal Disease Caused by a Variant of Neisseria meningitidis Serotype 2a ET-15 in a Community of Men Who Have Sex with Men. J. Clin. Microbiol. 41: 4411-4414 [Abstract] [Full Text]  
  • Wedege, E., Kuipers, B., Bolstad, K., van Dijken, H., Froholm, L. O., Vermont, C., Caugant, D. A., van den Dobbelsteen, G. (2003). Antibody Specificities and Effect of Meningococcal Carriage in Icelandic Teenagers Receiving the Norwegian Serogroup B Outer Membrane Vesicle Vaccine. Infect. Immun. 71: 3775-3781 [Abstract] [Full Text]  
  • Urwin, R., Holmes, E. C., Fox, A. J., Derrick, J. P., Maiden, M. C. J. (2002). Phylogenetic Evidence for Frequent Positive Selection and Recombination in the Meningococcal Surface Antigen PorB. Mol Biol Evol 19: 1686-1694 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Duh, R.-W., Singh, K. V., Murray, B. E. (2002). Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 868-876 [Abstract] [Full Text]  
  • Tondella, M. L. C., Popovic, T., Rosenstein, N. E., Lake, D. B., Carlone, G. M., Mayer, L. W., Perkins, B. A., The Active Bacterial Core Surveillance Team, (2000). Distribution of Neisseria meningitidis Serogroup B Serosubtypes and Serotypes Circulating in the United States. J. Clin. Microbiol. 38: 3323-3328 [Abstract] [Full Text]  
  • Urwin, R., Fox, A. J., Musilek, M., Kriz, P., Maiden, M. C. J. (1998). Heterogeneity of the PorB Protein in Serotype 22 Neisseria meningitidis. J. Clin. Microbiol. 36: 3680-3682 [Abstract] [Full Text]  
  • Sacchi, C. T., Lemos, A. P. S., Brandt, M. E., Whitney, A. M., Melles, C. E. A., Solari, C. A., Frasch, C. E., Mayer, L. W. (1998). Proposed Standardization of Neisseria meningitidis PorA Variable-Region Typing Nomenclature. CVI 5: 845-855 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»