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Clinical and Diagnostic Laboratory Immunology, May 1998, p. 404-406, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Primary Epitopes of Chicken Egg Yolk Antibodies to Peptidophosphogalactomannandagger

Brigitte A. Tuekam,Dagger Sandra J. Bonetti,§ and John E. Gander*

Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611-0700

Received 11 July 1997/Returned for modification 1 October 1997/Accepted 5 February 1998

Egg yolks from hens immunized with peptidophosphogalactomannan (pPGalManii), which contains 10 phosphocholine diester residues and is secreted by Penicillium fellutanum, contain antibodies against 5-O-beta -D-galactofuranosyl epitopes. These epitopes were the only significant determinants in pPGalManii. Approximately 60-fold less pPGalManii (1.6 µM galactofuran chains) was required for 50% inhibition than galactofurano-oligosaccharides or pPGalMan containing two galactofuranosyl residues per chain.

* Corresponding author. Mailing address: 4219 Rancho Grande Pl., NW, Albuquerque, NM 87120-5337. Phone: (505) 898-4128.

dagger This is Florida Agricultural Experiment Station Journal Series no. F-03326.

Dagger Present address: Jamaica Broilers Group, Best Dressed Chicken Division, Kingston 10, Jamaica.

§ Present address: Department of Chemistry, University of Southern Colorado, Pueblo, CO 81001-4901.

Clinical and Diagnostic Laboratory Immunology, May 1998, p. 404-406, Vol. 5, No. 3
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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