This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Granoff, D. M.
Right arrow Articles by Raff, H. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Granoff, D. M.
Right arrow Articles by Raff, H. V.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 479-485, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Modified Enzyme-Linked Immunosorbent Assay for Measurement of Antibody Responses to Meningococcal C Polysaccharide That Correlate with Bactericidal Responses

Dan M. Granoff,1,2,* Susan E. Maslanka,3 George M. Carlone,3 Brian D. Plikaytis,3 George F. Santos,1 Ahmad Mokatrin,1 and Howard V. Raff1

Chiron Vaccines, Emeryville,1 and Children's Hospital Oakland Research Institute, Oakland,2 California, and Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 30 December 1997/Returned for modification 3 March 1998/Accepted 2 April 1998

The standardized enzyme-linked immunosorbent assay (ELISA) for measurement of serum immunoglobulin G (IgG) antibody responses to meningococcal C polysaccharide has been modified to employ assay conditions that ensure specificity and favor detection primarily of high-avidity antibodies. The modified and standard assays were used to measure IgG antibody concentrations in sera of toddlers vaccinated with meningococcal polysaccharide vaccine or a meningococcal C conjugate vaccine. The results were compared to the respective complement-mediated bactericidal antibody titers. In sera obtained after one or two doses of vaccine, the correlation coefficients, r, for the results of the standard assay and bactericidal antibody titers were 0.45 and 0.29, compared to 0.85 and 0.87, respectively, for the modified assay. With the standard assay, there were no significant differences between the geometric mean antibody responses of the two vaccine groups. In contrast, with the modified assay, 5- to 20-fold higher postvaccination antibody concentrations were measured in the conjugate than in the polysaccharide group. Importantly, the results of the modified assay, but not the standard ELISA, paralleled the respective geometric mean bactericidal antibody titers. Thus, by employing conditions that favor detection of higher-avidity IgG antibody, the modified ELISA provides results that correlate closely with measurements of antibody functional activity that are thought to be important in protection against meningococcal disease.

* Corresponding author. Mailing address: Chiron Vaccines, 4560 Horton Street, R-311, Emeryville, CA 94608-2916. Phone: (510) 923-3467. Fax: (510) 923-4265. E-mail: dan_granoff{at}cc.chiron.com.

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 479-485, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baraldo, K., Mori, E., Bartoloni, A., Norelli, F., Grandi, G., Rappuoli, R., Finco, O., Del Giudice, G. (2005). Combined Conjugate Vaccines: Enhanced Immunogenicity with the N19 Polyepitope as a Carrier Protein. Infect. Immun. 73: 5835-5841 [Abstract] [Full Text]  
  • Aaberge, I. S., Oster, P., Helland, O. S., Kristoffersen, A.-C., Ypma, E., Hoiby, E. A., Feiring, B., Nokleby, H. (2005). Combined Administration of Meningococcal Serogroup B Outer Membrane Vesicle Vaccine and Conjugated Serogroup C Vaccine Indicated for Prevention of Meningococcal Disease Is Safe and Immunogenic. CVI 12: 599-605 [Abstract] [Full Text]  
  • Baraldo, K., Mori, E., Bartoloni, A., Petracca, R., Giannozzi, A., Norelli, F., Rappuoli, R., Grandi, G., Del Giudice, G. (2004). N19 Polyepitope as a Carrier for Enhanced Immunogenicity and Protective Efficacy of Meningococcal Conjugate Vaccines. Infect. Immun. 72: 4884-4887 [Abstract] [Full Text]  
  • Garcia-Ojeda, P. A., Hardy, S., Kozlowski, S., Stein, K. E., Feavers, I. M. (2004). Surface Plasmon Resonance Analysis of Antipolysaccharide Antibody Specificity: Responses to Meningococcal Group C Conjugate Vaccines and Bacteria. Infect. Immun. 72: 3451-3460 [Abstract] [Full Text]  
  • Harris, S. L., Finn, A., Granoff, D. M. (2003). Disparity in Functional Activity between Serum Anticapsular Antibodies Induced in Adults by Immunization with an Investigational Group A and C Neisseria meningitidis-Diphtheria Toxoid Conjugate Vaccine and by a Polysaccharide Vaccine. Infect. Immun. 71: 3402-3408 [Abstract] [Full Text]  
  • Harris, S. L., King, W. J., Ferris, W., Granoff, D. M. (2003). Age-Related Disparity in Functional Activities of Human Group C Serum Anticapsular Antibodies Elicited by Meningococcal Polysaccharide Vaccine. Infect. Immun. 71: 275-286 [Abstract] [Full Text]  
  • Mountzouros, K. T., Belanger, K. A., Howell, A. P., Bixler, G. S. Jr., Madore, D. V. (2002). A Glycoconjugate Vaccine for Neisseria meningitidis Induces Antibodies in Human Infants That Afford Protection against Meningococcal Bacteremia in a Neonate Rat Challenge Model. Infect. Immun. 70: 6576-6582 [Abstract] [Full Text]  
  • Baudner, B. C., Balland, O., Giuliani, M. M., Von Hoegen, P., Rappuoli, R., Betbeder, D., Del Giudice, G. (2002). Enhancement of Protective Efficacy following Intranasal Immunization with Vaccine Plus a Nontoxic LTK63 Mutant Delivered with Nanoparticles. Infect. Immun. 70: 4785-4790 [Abstract] [Full Text]  
  • Burrage, M., Robinson, A., Borrow, R., Andrews, N., Southern, J., Findlow, J., Martin, S., Thornton, C., Goldblatt, D., Corbel, M., Sesardic, D., Cartwight, K., Richmond, P., Miller, E. (2002). Effect of Vaccination with Carrier Protein on Response to Meningococcal C Conjugate Vaccines and Value of Different Immunoassays as Predictors of Protection. Infect. Immun. 70: 4946-4954 [Abstract] [Full Text]  
  • Santos, G. F., Deck, R. R., Donnelly, J., Blackwelder, W., Granoff, D. M. (2001). Importance of Complement Source in Measuring Meningococcal Bactericidal Titers. CVI 8: 616-623 [Abstract] [Full Text]  
  • Plested, J. S., Ferry, B. L., Coull, P. A., Makepeace, K., Lehmann, A. K., MacKinnon, F. G., Griffiths, H. G., Herbert, M. A., Richards, J. C., Moxon, E. R. (2001). Functional Opsonic Activity of Human Serum Antibodies to Inner Core Lipopolysaccharide (galE) of Serogroup B Meningococci Measured by Flow Cytometry. Infect. Immun. 69: 3203-3213 [Abstract] [Full Text]  
  • MAcLENNAN, J (2001). Meningococcal group C conjugate vaccines. Arch. Dis. Child. 84: 383-386 [Full Text]  
  • Richmond, P., Borrow, R., Findlow, J., Martin, S., Thornton, C., Cartwright, K., Miller, E. (2001). Evaluation of De-O-Acetylated Meningococcal C Polysaccharide-Tetanus Toxoid Conjugate Vaccine in Infancy: Reactogenicity, Immunogenicity, Immunologic Priming, and Bactericidal Activity against O-Acetylated and De-O-Acetylated Serogroup C Strains. Infect. Immun. 69: 2378-2382 [Abstract] [Full Text]  
  • Sikkema, D. J., Friedman, K. E., Corsaro, B., Kimura, A., Hildreth, S. W., Madore, D. V., Quataert, S. A. (2000). Relationship between Serum Bactericidal Activity and Serogroup-Specific Immunoglobulin G Concentration for Adults, Toddlers, and Infants Immunized with Neisseria meningitidis Serogroup C Vaccines. CVI 7: 764-768 [Abstract] [Full Text]  
  • Carson, P. J., Nichol, K. L., O'Brien, J., Hilo, P., Janoff, E. N. (2000). Immune Function and Vaccine Responses in Healthy Advanced Elderly Patients. Arch Intern Med 160: 2017-2024 [Abstract] [Full Text]  
  • MacLennan, J. M., Shackley, F., Heath, P. T., Deeks, J. J., Flamank, C., Herbert, M., Griffiths, H., Hatzmann, E., Goilav, C., Moxon, E. R. (2000). Safety, Immunogenicity, and Induction of Immunologic Memory by a Serogroup C Meningococcal Conjugate Vaccine in Infants: A Randomized Controlled Trial. JAMA 283: 2795-2801 [Abstract] [Full Text]  
  • Zhang, Q., Choo, S., Everard, J., Jennings, R., Finn, A. (2000). Mucosal Immune Responses to Meningococcal Group C Conjugate and Group A and C Polysaccharide Vaccines in Adolescents. Infect. Immun. 68: 2692-2697 [Abstract] [Full Text]  
  • MacDonald, N. E., Halperin, S. A., Law, B. J., Forrest, B., Danzig, L. E., Granoff, D. M. (1998). Induction of Immunologic Memory by Conjugated vs Plain Meningococcal C Polysaccharide Vaccine in Toddlers: A Randomized Controlled Trial. JAMA 280: 1685-1689 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»