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Clinical and Diagnostic Laboratory Immunology, July 1998, p. 537-542, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Potential Diagnostic and Vaccine Candidates of Helicobacter pylori by Two-Dimensional Gel Electrophoresis, Sequence Analysis, and Serum Profiling

C. Patrick McAtee,1,* Moon Young Lim,1 Kevin Fung,1 Mark Velligan,1 Kirk Fry,1 Theresa Chow,2 and Douglas E. Berg3

Genelabs Technologies, Inc., Redwood City, California 940631; Genelabs Diagnostics, Ltd., Singapore 0511, Singapore2; and Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 631103

Received 23 January 1998/Returned for modification 13 April 1998/Accepted 11 May 1998

There is great interest in characterizing the proteins of the gastric pathogen Helicobacter pylori, especially those to which humans respond immunologically, because of the potential importance of such proteins in diagnosis and vaccine development. Two-dimensional gel electrophoresis was used to separate and identify potential antigens of H. pylori ATCC 43504. Over 30 proteins were reactive in Western blots with pooled sera from 14 infected patients. These proteins were analyzed by N-terminal sequence analysis. Fourteen proteins were determined to be distinct from any proteins previously described from H. pylori; the others were previously isolated and characterized proteins. Analysis of eight distinct H. pylori strains showed that most of these antigens were produced by all of the strains. We propose that collection of new antigens such as those recognized here will be useful in serologic tests for detecting and monitoring H. pylori infection and may also serve as potential targets for antimicrobial agent or vaccine development.

* Corresponding author. Present address: Bristol-Myers Squibb Pharmaceutical Research Institute, P.O. Box 4000, Princeton, NJ 08543-4000. 

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 537-542, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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