This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Park, S. W. Articles by Griffin, D. E. Search for Related Content PubMed PubMed Citation Articles by Park, S. W. Articles by Griffin, D. E.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 583-587, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of Adhesion Molecules and CD28 on T Lymphocytes during Human Immunodeficiency Virus Infection

Suk W. Park,¹ Walter Royal III,¹ Richard D. Semba,^2,* Gordon W. Wiegand,³ and Diane E. Griffin^1,4

Departments of Neurology,¹ Ophthalmology,² and Medicine,³ School of Medicine, and Department of Molecular Microbiology and Immunology,⁴ School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland

Received 7 April 1997/Returned for modification 6 February 1998/Accepted 3 April 1998

Adhesion molecules, which play a major role in lymphocyte circulation, have not been well characterized in human immunodeficiency virus (HIV) infection. T-lymphocyte populations, including CD3, CD4, CD28, and adhesion molecules (L selectin, LFA-1, VLA-4, and ICAM-1) were measured by flow cytometry in a cross-sectional study of 100 HIV-infected and 49 HIV-seronegative adults. HIV-infected adults had lower numbers of CD3⁺ lymphocytes expressing L selectin (P < 0.0001) and VLA-4 (P < 0.01) and higher numbers of CD3⁺ lymphocytes expressing LFA-1^bright (P < 0.002) than did HIV-negative adults. By CD4⁺-lymphocyte count category (>500, 200 to 500, or <200 cells/µl), HIV-infected adults with more advanced disease had lower percentages of CD3⁺ lymphocytes expressing L selectin and VLA-4 and higher percentages of CD3⁺ lymphocytes expressing LFA-1. The percentages of CD3⁺ CD28⁺ lymphocytes and of CD3⁺ L selectin⁺ lymphocytes were positively correlated (Spearman coefficient = 0.86; P < 0.0001), and the percentage of CD3⁺ CD28⁺ lymphocytes and the CD3⁺ LFA-1^bright lymphocyte/CD3⁺ LFA-1^dim lymphocyte ratio were negatively correlated (Spearman coefficient = 0.92; P <0.00001). The results of this study suggest that HIV infection is associated with altered expression of adhesion molecules.

---

^* Corresponding author. Mailing address: Ocular Immunology Service, Suite 700, 550 North Broadway, Baltimore, MD 21205. Phone: (410) 955-3572. Fax: (410) 955-0629. E-mail: rdsemba{at}welchlink.welch.jhu.edu.

---

Clinical and Diagnostic Laboratory Immunology, July 1998, p. 583-587, Vol. 5, No. 4
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Sipsas, N. V., Sfikakis, P. P. (2004). Expanding Role of Circulating Adhesion Molecules in Assessing Prognosis and Treatment Response in Human Immunodeficiency Virus Infection. CVI 11: 996-1001 [Full Text]  
• Chen, J. J-Y., Huang, J. C., Shirtliff, M., Briscoe, E., Ali, S., Cesani, F., Paar, D., Cloyd, M. W. (2002). CD4 lymphocytes in the blood of HIV+ individuals migrate rapidly to lymph nodes and bone marrow: support for homing theory of CD4 cell depletion. J. Leukoc. Biol. 72: 271-278 [Abstract] [Full Text]  
• Marschner, S., Freiberg, B. A., Kupfer, A., Hunig, T., Finkel, T. H. (1999). Ligation of the CD4 receptor induces activation-independent down-regulation of L-selectin. Proc. Natl. Acad. Sci. USA 96: 9763-9768 [Abstract] [Full Text]