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Clinical and Diagnostic Laboratory Immunology, September 1998, p. 613-616, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

Felix Grimm,* Friedrich E. Maly,dagger Jian Lü,Dagger and Roberto Llano

Institute of Parasitology, University of Zurich, Zürich, Switzerland

Received 29 September 1997/Returned for modification 11 February 1998/Accepted 10 June 1998

The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

* Corresponding author. Mailing address: Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland. Phone: (01) 635 85 05: Fax: (01) 635 89 15. E-mail: fgrimm{at}vetparas.unizh.ch.

dagger Present address: Institute of Clinical Chemistry, University Hospital Zurich, CH-8091 Zürich, Switzerland.

Dagger Present address: Department of General Surgery, The First Affiliated Hospital, Xinjiang Medical College, Urumqi, Xinjiang 83000, China.

Clinical and Diagnostic Laboratory Immunology, September 1998, p. 613-616, Vol. 5, No. 5
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Eckert, J., Deplazes, P. (2004). Biological, Epidemiological, and Clinical Aspects of Echinococcosis, a Zoonosis of Increasing Concern. Clin. Microbiol. Rev. 17: 107-135 [Abstract] [Full Text]  


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