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Clinical and Diagnostic Laboratory Immunology, November 1998, p. 749-754, Vol. 5, No. 6
Departamento de Microbiología,
Received 23 March 1998/Returned for modification 8 May
1998/Accepted 23 July 1998
Brucella abortus and Brucella melitensis
have surface lipopolysaccharides and polysaccharides carrying
B. melitensis-type (M) and B. abortus-type (A) epitopes as well as common (C) epitopes present
in all smooth Brucella biotypes. Crude lipopolysaccharides, hydrolytic O polysaccharides, and native hapten polysaccharides of MC
or AC specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein G conjugates by using sera from cattle, sheep, and goats infected with
AC, MC, or AMC Brucella biotypes. Regardless of the
antigen, the levels of antibodies were lower in goats than in sheep and highest in cattle. The diagnostic performance of the assay was not
affected by the absence of lipid A-core epitopes, the presence of
contaminating outer membrane proteins, the AC or MC epitopic structure
of the absorbed antigen, or the conjugate used. Moreover, with sera
from cattle vaccinated with B. abortus S19 (AC) or
from sheep and goats vaccinated with B. melitensis Rev
1 (MC), AC and MC antigens showed similar levels of reactivity. The
results show that antibodies to the C epitopes largely dominate in
infection, and this is consistent with the existence of
multiple overlapping C epitopes (V. Weynants, D. Gilson, A. Cloeckaert,
A. Tibor, P. A. Denoel, F. Godfroid, J. N. Limet, and J.-J.
Letesson, Infect. Immun. 65:1939-1943, 1997) rather than with one or
two C epitopes. It is concluded that, by adaptation to the
corresponding antibody levels, brucellosis in cattle, sheep, and goats
can be diagnosed by immunosorbent assay with a single combination of
conjugate and antigen.
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Evaluation of Lipopolysaccharides and Polysaccharides of
Different Epitopic Structures in the Indirect Enzyme-Linked
Immunosorbent Assay for Diagnosis of Brucellosis in Small Ruminants
and Cattle
*
Corresponding author. Mailing address: Departamento de
Microbiología, Universidad de Navarra, Aptdo. 177, 31080 Pamplona, Spain. Phone: 34-948-425600. Fax: 34-948-425649. E-mail:
imoriyon{at}unav.es.
Clinical and Diagnostic Laboratory Immunology, November 1998, p. 749-754, Vol. 5, No. 6
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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