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Clinical and Diagnostic Laboratory Immunology, November 1998, p. 773-779, Vol. 5, No. 6
Department of Pathobiology, Ontario
Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1,
Canada,1 and
Department of Veterinary
Science, South Dakota State University, Brookings, South Dakota
570072
Received 15 May 1998/Returned for modification 7 August
1998/Accepted 21 August 1998
A collection of 12 monoclonal antibodies (MAbs) raised against
porcine reproductive and respiratory syndrome (PRRS) virus was used to
study the antigenic structure of the virus nucleocapsid protein (N).
The full-length N gene, encoded by open reading frame 7, was cloned
from the Canadian PRRS virus, PA-8. Deletions were introduced into the
N gene to produce a series of nine overlapping protein fragments
ranging in length from 25 to 112 amino acids. The individual truncated
genes were cloned as glutathione S-transferase fusions into
a eukaryotic expression vector downstream of the T7 RNA polymerase
promoter. HeLa cells infected with recombinant vaccinia virus
expressing T7 RNA polymerase were transfected with plasmid DNA encoding
the N protein fragments, and the antigenicity of the synthesized
proteins was analyzed by immunoprecipitation. Based on the
immunoreactivities of the N protein deletion mutants with the panel of
N-specific MAbs, five domains of antigenic importance were identified.
MAbs SDOW17, SR30, and 5H2.3B12.1C9 each identified independent domains
defined by amino acids 30 to 52, 69 to 123, and 37 to 52, respectively.
Seven of the MAbs tested specifically recognized the local protein
conformation formed in part by the amino acid residues 52 to 69. Furthermore, deletion of 11 amino acids from the carboxy terminus of
the nucleocapsid protein disrupted the epitope configuration recognized
by all of the conformation-dependent MAbs, suggesting that the
carboxy-terminal region plays an important role in maintaining local
protein conformation.
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Antigenic Structure of the Nucleocapsid Protein of
Porcine Reproductive and Respiratory Syndrome Virus
*
Corresponding author. Mailing address: Department of
Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Phone: (519) 824-4120, ext. 4729. Fax: (519)
767-0809. E-mail: dyoo{at}ovcnet.uoguelph.ca.
Clinical and Diagnostic Laboratory Immunology, November 1998, p. 773-779, Vol. 5, No. 6
1071-412X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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