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Clinical and Diagnostic Laboratory Immunology, March 1999, p. 168-172, Vol. 6, No. 2
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development of a Western Blot Assay for Detection of Bovine Immunodeficiency-Like Virus Using Capsid and Transmembrane Envelope Proteins Expressed from Recombinant Baculovirus

Y. Abed,1 G. St-Laurent,1 H. Zhang,1 R. M. Jacobs,2 and D. Archambault1,*

Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec,1 and Department of Pathobiology, University of Guelph, Guelph, Ontario,2 Canada

Received 22 July 1998/Returned for modification 15 September 1998/Accepted 9 November 1998

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.

* Corresponding author. Mailing address: Université du Québec à Montréal, Département des Sciences Biologiques, C. P. 8888, Succursale Centre-Ville, Montréal, Québec, Canada H3C 3P8. Phone: (514) 987-3000, ext. 4622. Fax: (514) 987-4647. E-mail: archambault.denis{at}uqam.ca.

Clinical and Diagnostic Laboratory Immunology, March 1999, p. 168-172, Vol. 6, No. 2
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jeronimo, C., Archambault, D. (2002). Importance of M-Protein C Terminus as Substrate Antigen for Serodetection of Equine Arteritis Virus Infection. CVI 9: 698-703 [Abstract] [Full Text]  


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