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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 489-493, Vol. 6, No. 4
Division of Infectious
Diseases1 and Division of
Gastroenterology,
Received 28 October 1998/Returned for modification 8 February
1999/Accepted 15 March 1999
Several different families of vacuolating toxin (vacA)
alleles are present in Helicobacter pylori, and they encode
products with differing functional activities. H. pylori
strains containing certain types of vacA alleles have been
associated with an increased risk for peptic ulcer disease. In this
study, we tested serum samples and gastric juice from 19 H. pylori-negative and 39 H. pylori-positive patients
for enzyme-linked immunosorbent assay reactivity with two different
types of VacA antigens (types s1/m1 and s2/m2), which were purified
from H. pylori 60190 and 86-338, respectively. Both
antigens were recognized better by serum immunoglobulin G (IgG) from
H. pylori-positive persons than by serum IgG from H. pylori-negative persons (P < 0.01). The s1/m1
VacA antigen was better recognized by sera from patients carrying
vacA type s1/m1 strains than by sera from patients carrying
vacA type s2/m2 or s1/m2 strains (P < 0.01). Conversely, the s2/m2 VacA antigen was better recognized by sera
from patients carrying type s2/m2 or s1/m2 strains (P = 0.03). Serum IgG anti-VacA antibodies were present more frequently in
patients carrying type s1/m1 strains than in other H. pylori-positive patients (P = 0.0002). In
addition, the highest levels of IgA anti-VacA antibodies were detected
in the gastric juice of patients carrying type s1/m1 strains. These data indicate that different VacA isoforms have distinct antigenic properties and that multiple forms of VacA elicit antibody responses in
H. pylori-positive humans.
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Detection of Anti-VacA Antibody Responses in Serum
and Gastric Juice Samples Using Type s1/m1 and s2/m2
Helicobacter pylori VacA Antigens
*
Corresponding author. Mailing address: Division of
Infectious Diseases, A3310 MCN, Vanderbilt University School of
Medicine, Nashville, TN 37232. Phone: (615) 322-2035. Fax: (615)
343-6160. E-mail: COVERTL{at}ctrvax.vanderbilt.edu.
Present address: Division of Gastroenterology, University Hospital,
Queen's Medical Centre, Nottingham, England.
Clinical and Diagnostic Laboratory Immunology, July 1999, p. 489-493, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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