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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 519-524, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Pneumococcal Capsular Polysaccharide Preparations May Contain Non-C-Polysaccharide Contaminants That Are Immunogenic

Xinhong Yu,1 Yan Sun,1 Carl Frasch,2 Nelydia Concepcion,2 and Moon H. Nahm1,3,4,*

Departments of Pediatrics,1 Pathology,3 and Medicine,4 University of Rochester School of Medicine, Rochester, New York 14642, and Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 208532

Received 7 December 1998/Returned for modification 18 February 1999/Accepted 15 March 1999

We measured the capacity to opsonize Streptococcus pneumoniae serotype 6B and estimated the concentration of immunoglobulin G anti-6B capsular polysaccharide (PS) antibodies in 25 pre- and postimmune sera from adults immunized with a pneumococcal PS vaccine. We first studied two postvaccination serum samples displaying less opsonophagocytic capacity than expected. The majority of anti-6B antibodies in the two samples reacted with the capsular PSs of several unrelated serotypes (2, 4, 9V, 19F, and 23F) and with the lysate of noncapsulated S. pneumoniae bacteria but not with C-PS. The non-type-specific antibodies accounted for at least one-half of anti-6B antibodies in 40% of prevaccination sera and 10% of postvaccination sera from adults. The non-type-specific antibodies could be demonstrated in the enzyme-linked immunosorbent assays (ELISAs) for pneumococcal antibodies to other serotypes (4, 9V, 18C, 19F, and 23F). The nonspecific antibodies appear to bind a contaminant(s) in the current preparations of capsular PS. ELISA for antibodies to pneumococcal capsules may not be serotype specific for some samples.

* Corresponding author. Mailing address: Department of Pediatrics, University of Rochester, 601 Elmwood Ave., Box 777, Rochester, NY 14642. Phone: (716) 275-7963. Fax: (716) 271-7512. E-mail: moon{at}vaccine.rochester.edu.

Clinical and Diagnostic Laboratory Immunology, July 1999, p. 519-524, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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