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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 633-638, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serum Antibody Responses to Helicobacter pylori and the cagA Marker in Patients with Mucosa-Associated Lymphoid Tissue Lymphoma

Anne Taupin,1 Alessandra Occhialini,1 Agnès Ruskone-Fourmestraux,2 Jean-Charles Delchier,3 Jean-Claude Rambaud,4 and Francis Mégraud1,*

Laboratoire de Bactériologie, Université de Bordeaux 2, 33076 Bordeaux Cedex,1 Service de Gastroentérologie, Hôtel-Dieu de Paris, 75181 Paris Cedex 4,2 Service d'Hépatogastroentérologie, Hôpital Henri Mondor, 94010 Créteil,3 and Service de Gastroénterologie, Hôpital Lariboisière, 75475 Paris cedex 10,4 France

Received 5 August 1998/Returned for modification 28 December 1998/Accepted 13 April 1999

The lymphoma of the mucosa-associated lymphoid tissue (MALT) of the stomach has been linked to Helicobacter pylori infection, but the mechanisms involved in B-cell proliferation remain elusive. In a search for putative H. pylori-specific monoclonal immunoglobulin production, an H. pylori strain was isolated from 10 patients with MALT lymphoma and used to detect the specific serum antibody response to the homologous strain by immunoblotting. Moreover, the antigenicity of the different strains was compared by using each of the 10 sera. We found that the different strains induced highly variable patterns of systemic immunoglobulin G antibody response, although several bacterial antigens, such as the 60-kDa urease B, were often recognized by the different sera. The cagA marker was detected in the strains by PCR with specific primers and by dot blot analysis, and the CagA protein was found in the sera of 4 of the 10 patients by immunoblotting. In conclusion, MALT lymphoma patients, like other patients with H. pylori gastritis, exhibit a polymorphic systemic antibody response, despite an apparently similar antigenic profile. The CagA marker of pathogenicity is not associated with this disease.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Université de Bordeaux 2, 33076 Bordeaux Cedex, France. Phone: (33) 5-56-79-59-10. Fax: (33) 5-56-79-60-18. E-mail: francis.megraud{at}chu-aquitaine.fr.

Clinical and Diagnostic Laboratory Immunology, July 1999, p. 633-638, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lehours, P., Menard, A., Dupouy, S., Bergey, B., Richy, F., Zerbib, F., Ruskone-Fourmestraux, A., Delchier, J. C., Megraud, F. (2004). Evaluation of the Association of Nine Helicobacter pylori Virulence Factors with Strains Involved in Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma. Infect. Immun. 72: 880-888 [Abstract] [Full Text]  
  • Zucca, E., Bertoni, F., Roggero, E., Cavalli, F. (2000). The gastric marginal zone B-cell lymphoma of MALT type. Blood 96: 410-419 [Full Text]  


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