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Clinical and Diagnostic Laboratory Immunology, November 1999, p. 787-790, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Complement Fixation Test To Assess Humoral Immunity in Cattle and Sheep Vaccinated with Brucella abortus RB51

Rosanna Adone* and Franco Ciuchini

Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanità, 00161 Rome, Italy

Received 12 March 1999/Returned for modification 28 June 1999/Accepted 26 July 1999

The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. abortus RB51 infection in humans.

* Corresponding author. Mailing address: Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-6-49902728. Fax: 39-6-49387077. E-mail: adone{at}iss.it.

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 787-790, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Adone, R., Ciuchini, F., Marianelli, C., Tarantino, M., Pistoia, C., Marcon, G., Petrucci, P., Francia, M., Riccardi, G., Pasquali, P. (2005). Protective Properties of Rifampin-Resistant Rough Mutants of Brucella melitensis. Infect. Immun. 73: 4198-4204 [Abstract] [Full Text]  
  • Pasquali, P., Rosanna, A., Pistoia, C., Petrucci, P., Ciuchini, F. (2003). Brucella abortus RB51 Induces Protection in Mice Orally Infected with the Virulent Strain B. abortus 2308. Infect. Immun. 71: 2326-2330 [Abstract] [Full Text]  
  • Ciuchini, F., Adone, R., Pasquali, P. (2002). Coombs Antiglobulin Test Using Brucella abortus 99 as Antigen To Detect Incomplete Antibodies Induced by B. abortus RB51 Vaccine in Cattle. CVI 9: 1398-1399 [Abstract] [Full Text]  
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