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Clinical and Diagnostic Laboratory Immunology, November 1999, p. 953-958, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison and Characterization of Immunoglobulin G Subclasses among Primate Species

Michael H. Shearer,1 Robyn D. Dark,2 James Chodosh,3 and Ronald C. Kennedy1,*

Departments of Microbiology and Immunology, University of Oklahoma Health Sciences Center,1 and Ophthalmology and Molecular Pathogenesis of Eye Infection Research Center, Dean A. McGee Eye Institute,3 Oklahoma City, Oklahoma 73104, and East Central University, Department of Biology, Ada, Oklahoma 748202

Received 10 May 1999/Returned for modification 24 June 1999/Accepted 18 August 1999

Little information is available on the immunoglobulin G (IgG) subclasses expressed in the sera of nonhuman primate species. To address this issue, we compared the IgG subclasses found in humans (IgG1, IgG2, IgG3, and IgG4) to those of nonhuman primates, such as baboons and macaques. Cross-reactive antihuman IgG subtype-specific reagents were identified and used to analyze purified IgG from sera by solid-phase enzyme-linked immunosorbent assay. Protein A-purified human IgG obtained from sera was composed of IgG1, IgG2, IgG3, and IgG4, whereas baboon and macaque IgG was composed of IgG1, IgG2, and IgG4. Protein G-purified human IgG was composed of IgG1, IgG2, IgG3, and IgG4, whereas baboon and macaque IgG was composed of IgG1, IgG2, and IgG4. To test the possibility that baboon and macaque IgG3 is actually present, but is outcompeted for binding to proteins A and G by the other more abundant IgG subclasses, we repurified the IgG from sera that did not bind either protein A or protein G. We found a baboon IgG3 population in the sera that did not bind protein A, but bound protein G. No IgG3 subtype was detectable in macaque sera. These data suggest that baboon sera, like human sera, contain four IgG subtypes, whereas macaque sera exhibit only three of the human subclass analogs. In addition, the IgG subtype-specific reagents were shown to be useful in determining the IgG subclass distribution following vaccination of baboons with hepatitis B surface antigen.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 800 N. Research Parkway, Suite 462, Oklahoma City, OK 73104. Phone: (405) 271-5630. Fax: (405) 271-6339. E-mail: ronald-kennedy{at}ouhsc.edu.

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 953-958, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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