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Clinical and Diagnostic Laboratory Immunology, November 1999, p. 966-969, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antigenic Homology of the Inducible Ferric Citrate Receptor (FecA) of Coliform Bacteria Isolated from Herds with Naturally Occurring Bovine Intramammary Infections

Jun Lin,* Joseph S. Hogan, and K. Larry Smith

Department of Animal Sciences, Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691

Received 29 April 1999/Returned for modification 10 August 1999/Accepted 7 September 1999

Expression of ferric citrate receptor FecA by Escherichia coli and Klebsiella pneumoniae isolated from bovine mastitis was investigated. Transformant E. coli UT5600/pSV66, which produces large quantities of FecA in the presence of citrate, was constructed. The FecA of E. coli UT5600/pSV66 was purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to prepare polyclonal antiserum in rabbits. All coliform isolates of E. coli (n = 18) and K. pneumoniae (n = 17) from naturally occurring bovine intramammary infections in five herds induced iron-regulated outer membrane proteins when grown in Trypticase soy broth containing 200 µM alpha -alpha '-dipyridyl and 1 mM citrate. Polyclonal antiserum against FecA was used in conjunction with an immunoblot technique to determine the degree of antigenic homology of FecA among isolates. In the presence of citrate, each isolate expressed FecA that reacted with the anti-FecA polyclonal antiserum. The molecular mass of FecA (~80.5 kDa) was also highly conserved among isolates. Therefore, the ferric citrate iron transport may be induced in coliform bacteria and utilized to acquire iron in milk for survival and growth. The FecA is an attractive vaccine component for controlling coliform mastitis during the lactation period.

* Corresponding author. Present address: Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, Ohio 44106-4965. Phone: (216) 368-6187. Fax: (216) 368-3395. E-mail: jxl99{at}po.cwru.edu.

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 966-969, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wolf, S. L., Hogan, J. S., Smith, K. L. (2004). Iron Uptake by Escherichia coli Cultured with Antibodies from Cows Immunized with High-Affinity Ferric Receptors. J DAIRY SCI 87: 2103-2107 [Abstract] [Full Text]  
  • Takemura, K., Hogan, J. S., Smith, K. L. (2004). Growth Responses of Escherichia coli to Immunoglobulin G from Cows Immunized with Ferric Citrate Receptor, FecA. J DAIRY SCI 87: 316-320 [Abstract] [Full Text]  
  • Takemura, K., Hogan, J. S., Smith, K. L. (2003). Effect of Immunoglobulin G from Cows Immunized with Ferric Citrate Receptor (FecA) on Iron Uptake by Escherichia coli. J DAIRY SCI 86: 133-137 [Abstract] [Full Text]  
  • Wise, A. J., Hogan, J. S., Takemura, K., Smith, K. L. (2003). Opsonic Activity of Serum and Whey from Cows Immunized with the Ferric Citrate Receptor. J DAIRY SCI 86: 146-151 [Abstract] [Full Text]  
  • Luck, S. N., Turner, S. A., Rajakumar, K., Sakellaris, H., Adler, B. (2001). Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes. Infect. Immun. 69: 6012-6021 [Abstract] [Full Text]  


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