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Clinical and Diagnostic Laboratory Immunology, November 1999, p. 983-985, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Increased Levels of epsilon  and gamma  Isoforms of 14-3-3 Proteins in Cerebrospinal Fluid in Patients with Creutzfeldt-Jakob Disease

Hidehiro Takahashi,1,* Toshinari Iwata,1 Yoshinori Kitagawa,1 Reisuke H. Takahashi,1 Yuko Sato,1 Hideki Wakabayashi,2 Miwa Takashima,2 Hiroshi Kido,2 Kazuo Nagashima,3,4 Kimbra Kenney,5 Clarence J. Gibbs Jr.,5 and Takeshi Kurata1

Department of Pathology, National Institute of Infectious Diseases, Tokyo,1 Division of Enzyme Chemistry, Institute of Enzyme Research, University of Tokushima, Tokushima,2 Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Hokkaido,3 and CREST-JST, Sapporo,4 Japan, and Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland5

Received 22 February 1999/Returned for modification 5 April 1999/Accepted 8 September 1999

We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that varepsilon  and gamma  isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.

* Corresponding author. Mailing address: Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640 Japan. Phone: 81-3-5285-1111, ext. 2625. Fax: 81-3-5285-1189. E-mail: htakahas{at}nih.go.jp.

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 983-985, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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