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 Previous Article

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 986-988, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

bca, Beta Gene, and Gene Product Divergency in Reference and Prototype Strains of Streptococcus agalactiae

Johan A. Maeland,* Lars Bevanger, Grethe Iversen, and Randi Valsoe Lyng

Department of Microbiology, School of Medicine, Norwegian University of Science and Technology, N-7006, Trondheim, Norway

Received 30 April 1999/Returned for modification 9 July 1999/Accepted 14 September 1999

Reference and prototype strains of Streptococcus agalactiae (GBS) were originally selected on the basis of phenotypic traits which, however, do not always mirror genotypic traits. A total of 14 reference and prototype GBS strains were examined by PCR designed to detect the bca and beta genes, encoding the c proteins calpha and cbeta , respectively. The cognate proteins were detected by whole-cell-based fluorescent antibody testing and Western blotting. The PCR for beta gene detection and the antibody-based cbeta protein detection showed concordant results with all of the isolates, whereas 7 of 14 strains which did not express calpha protein at detectable levels contained bca gene elements, consistent with bca gene and gene product divergency in these strains. The results emphasize the importance of genetic characterization of reference and prototype strains of GBS which, in the past, have been selected on the basis of phenotypic traits.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, Norwegian University of Science and Technology, N-7006, Trondheim, Norway. Phone: (47) 73 86 84 84. Fax: (47) 73 86 77 65.

Clinical and Diagnostic Laboratory Immunology, November 1999, p. 986-988, Vol. 6, No. 6
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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